His·Tag^?Monoclonal Antibody

半岛bd体育手机客户端 说明

一般描述

Sensitive, specific detection of His?Tag fusion proteins
The HIS TAG^?Monoclonal Antibody is a mouse monoclonal antibody (IgG₁) directed against the HIS TAG^?sequence encoded by many of the NOVAGEN^?expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (K_d= 5 × 10^-8to 1 × 10^-9M) enables sensitive, specific detection of HIS TAG^?fusion proteins at antibody concentrations of 0.1-0.2 g/ml. The HIS TAG^?Monoclonal Antibody binds to N-terminal, C-terminal, and internal HIS TAG^?sequences. This antibody also detects the recombinant marker bands in the Perfect Protein and Trail Mix Western Markers for convenient visualization of accurate internal standards on Western blots*. For lowest background in Western blotting applications, Alkali-soluble Casein is recommended as a blocking agent. The 100-g package size provides enough purified antibody for 1000 mL of working solution.
* Not recommended for HRP-based detection of Trail Mix Western Markers.

警告

Toxicity: Standard Handling (A)

法律信息

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
抗体形式	purified antibody
manufacturer/tradename	Novagen?
储存条件	OK to freeze
储存温度	2-8℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable