TIMP-1, Human, Recombinant, CHO Cells

半岛bd体育手机客户端 说明

一般描述

Recombinant, human TIMP-1 expressed in CHO cells. Migrates as a 28 kDa protein under reducing conditions on SDS-PAGE.
Recombinant, human TIMP-1 expressed in CHO cells. Migrates as a ~28 kDa protein by SDS-PAGE. The activation and activity of matrix metalloproteinases (MMPs) are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP-1 is a ~28 kDa glycoprotein that is expressed by a variety of cell types. It forms a non-covalent, stoichiometric complex with both latent and active MMPs. TIMP-1 preferentially binds and inhibits MMP-9. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models.

包装

3 μg in Plastic ampoule

应用

Immunoblotting Standard (20 ?l/lane)

MMP Inhibition (assay dependent, see comments)

生化/生理作用

IC?? against native human MMP-1 is ~1-5 nM.
Product does not compete with ATP.
Primary Target
Human MMP 1
Reversible: no
Target IC₅₀: 1-5 nM against human MMP 1
Cell permeable: no

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from ammonium bicarbonate, sodium chloride, sodium phosphate buffer, 100 ?g BSA, pH 7.3.

重悬

Reconstitute with PBS to the desired concentration and mix gently until completely dissolved.
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.

其他说明

Freudenstein, J. et al. 1990.Biochem. Biophys. Res. Comm.171,250.
Liotta, L. A., and Stetler-Stevenson, W. G. 1990. inSeminars in Cancer Biology, ed. M. M. Gottesman.Vol. 1,99.
Kodama, S. et al. 1987.Collagen Rel. Res.7,341.
Docherty, A.J.P. et al. 1985.Nature318,66.
Nagai, Y., et al. 1984.Jpn. J. Inflam.4,123.
TIMPs bind MMPs in a 1:1 stoichiometric ratio, but due to the variable concentration of MMPs in biological samples the optimal amount of TIMP-1 will be assay dependent and should be determined by titration. It is reported that TIMP-1 inhibits 6.7 nM MMP-1 with an IC₅₀of 1-5 nM (see application references).

法律信息

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
测定	≥95% (prior to lyophilization, SDS-PAGE)
形式	lyophilized
不包含	preservative
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable