Rosetta-gami B Competent Cell Set-Novagen

半岛bd体育手机客户端 说明

一般描述

Genotypes:

Rosetta-gami B

F^–ompT hsdS_B(r_B^–m_B^–)gal dcm lacY1 ahpC gor522::Tn10 trxBpRARE (Cam^R, Kan^R, Tet^R)



Rosetta-gami B(DE3)

F^–ompT hsdS_B(r_B^–m_B^–)gal dcm lacY1 ahpC(DE3)gor522::Tn10 trxBpRARE (Cam^R, Kan^R, Tet^R)



Rosetta-gami B(DE3)pLysS

F^–ompT hsdS_B(r_B^–m_B^–)gal dcm lacY1 ahpC(DE3)gor522::Tn10 trxBpLysSRARE (Cam^R, Kan^R, Tet^R)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Rosetta-gami Bstrains combine the key features of BL21 (and its Tuner derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm. These strains are compatible with ampicillin- or spectinomycin-resistant vectors.

DE3indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of thelacUV5promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysSstrains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.

组分

?2 × 0.2 mlRosetta-gami B Competent Cells

?2 × 0.2 mlRosetta-gami B(DE3) Competent Cells

?2 × 0.2 mlRosetta-gami B(DE3)pLysS Competent Cells

?4 × 2 mlSOC Medium

?2 ngTest Plasmid

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

法律信息

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

manufacturer/tradename	Novagen?
质量水平	100
储存条件	OK to freeze avoid repeated freeze/thaw cycles
储存温度	?70℃