pLysS DNA-Novagen

半岛bd体育手机客户端 说明

一般描述

pLysS and pLysE are 4886bp plasmids constructed by insertion of the T7 lysozyme gene into theBamH I site of pACYC184 (1). These plasmids are not cloning vectors; they are used in λDE3 lysogenic hosts to suppress basal expression from the T7 promoter by producing T7 lysozyme, a natural inhibitor of T7 RNA polymerase. The two plasmids differ only by the orientation of the T7 lysozyme gene. In pLysS the T7 lysozyme coding sequence is in the antisense orientation relative to thetetpromoter, so only a small amount of T7 lysozyme is produced. In pLysE large amounts of T7 lysozyme are produced from thetetpromoter. The construct also contains the weak T7 φ3.8 promoter immediately following the lysozyme gene. The p15A origin of replication is compatible with those found in pBR322- and pUC-derived plasmids.

This product is covered under license from Brookhaven National Labs.Commercial entitiesneed to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

包装

1 μg in Glass bottle

警告

Toxicity: Standard Handling (A)

其他说明

1. Studier , F.W. (1991)J. Mol. Biol.219, 37-44.

法律信息

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
形式	solid
manufacturer/tradename	Novagen?
储存条件	OK to freeze avoid repeated freeze/thaw cycles
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable