半岛bd体育手机客户端 说明
一般描述
The BCA protein assay is based on a biuretreaction, which is the reduction of Cu2+toCu+by proteins in an alkaline solution withconcentration-dependent detection of themonovalent copper ions. Bicinchoninic acidis a chromogenic reagent that chelates thereduced copper, producing a purple complexwith strong absorbance at 562 nm (Smith1985, Wiechelman 1988). This assay can beused to quantify protein concentration with awide variety of samples and can be performedin minutes.
The Novagen?BCA Protein Assay Kit canbe used to determine protein concentrationin the range of 20-2000 ?g/ml in either astandard assay or microassay configuration.Kit components are sufficient to complete500 standard-size reactions (50 ?l proteinsample plus 1 ml reagent) or 2500 micro-scalereactions (25 ?l protein sample plus 200 ?lreagent). A BSA standard (2 mg/ml) is providedfor convenient and reliable preparation ofstandard curves.
This assay is robust and can be performedin the presence of many compounds. Somereagents, including chelating agents, strongacids or bases, and reducing agents, interferewith the reduction and chelating reactions onwhich this assay depends (Brown 1989). TheBCA assay is compatible with the followingNovagen protein extraction and lysis reagents:BugBuster
The BCA protein assay is a simple and reliable protein quantification method.
组分
?500 mlBCA Solution
?15 ml4% Cupric Sulfate
?3 x 1 mlBSA Standard, 2 mg/ml
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
其他说明
Smith, P.K., et al. 1985.Anal. Biochem.150, 76.
Kessler, R. J. and Fanestil, D. D. 1986. Anal. Biochem159, 138.
Wiechelman, K., et al. 1988.Anal. Biochem.175, 231.
Brown, R., et al. 1989.Anal. Biochem.180, 136.
法律信息
Use of the BCA protein assay is permitted for research purposes only.
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
| manufacturer/tradename | Novagen? |
| 储存条件 | OK to freeze |
| 储存温度 | 10-30℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
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