Anti-TRA-1-81 Antibody, clone TRA-1-81, Cy3 conjugate

半岛bd体育手机客户端 说明

一般描述

TRA-1-81 is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). Both TRA-1-81 and TRA-1-60 recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope while TRA-1-81 reacts with an unknown epitope of the same molecule. TRA-1-81 is commonly used as marker of undifferentiated pluripotent human stem cells and is downregulated upon differentiation. TRA-1-81 has recently been identified as podocalyxin.

特异性

This antibody recognizes human TRA-1-81.

免疫原

Human embryonal carcinoma cell line 2102Ep

应用

Evaluated by Immunocytochemistry in mouse embryonic stem cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody did not detect TRA-1-81 in mouse embryonic stem cells (SCR012).
This Anti-TRA-1-81 Antibody, clone TRA-1-81, Cy3 conjugate is validated for use in ICC for the detection of TRA-1-81.
Research Sub Category
Pluripotent & Early Differentiation
Research Category
Stem Cell Research

质量

Evaluated by Immunocytochemistry in H9 human embryonic stem cells.
Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected TRA-1-81 in H9 human embryonic stem cells.

目标描述

420/250 kDa calculated

外形

Ammonium sulfate precipitation followed by size exclusion.
Purified mouse monoclonal IgM conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.

储存及稳定性

Maintain refrigerated at 2-8?C protected from light in undiluted aliquots for up to 6 months from date of receipt.

分析说明

Control
H9 human embryonic stem cells

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
偶联物	CY3 conjugate
抗体形式	saturated ammonium sulfate (SAS) precipitated
antibody product type	primary antibodies
克隆	TRA-1-81, monoclonal
species reactivity	human
technique(s)	immunocytochemistry: suitable
同位素/亚型	IgM
NCBI登记号	NP_001018121.1
UniProt登记号	O00592
运输	wet ice
Gene Information	human ... PODXL(5420)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable