半岛bd体育手机客户端 说明

一般描述

The Human Fas Ligand ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Fas Ligand in serum, plasma, cell culture supernatants and urine.

免疫原

Recombinant Human Fas Ligand

应用

请参考Protocol了解详情。

生化/生理作用

Binding of Fas Ligand (FasL) to Fas receptor triggers apoptosis in Fas-bearing cells. FasL has the ability to kill T cells and activate B cells which leads to down-regulation of the immune response. The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD (Fas-associated protein with death domain) followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to eventual cell death. FasL is also involved in AGE (advanced glycation end-product)-mediated apoptosis in human retinal ARPE-19 cells, suggesting its role in diabetic retinopathy. Changes in the activity of FasL suppress normal apoptosis, leading to abnormal survival and growth of tumor cells. Mutations in the FasL gene causes autoimmune lymphoproliferative syndrome (ALPS).

其他说明

A sample Certificate of Analysis is available for this product.
Please type the wordsamplein the text box provided for lot number.

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

species reactivity	human
包装	kit of 96 wells (12 strips x 8 wells)
technique(s)	ELISA: suitable capture ELISA: suitable
input	sample type plasma sample type cell culture supernatant(s) sample type serum
assay range	inter-assay cv: <12% intra-assay cv: <10% sensitivity: 2 pg/mL standard curve range: 1.37-1000 pg/mL
detection method	colorimetric
运输	wet ice
储存温度	?20℃
Gene Information	human ... FASLG(356)

组份列表

组份可单独销售

货号	组份
RABELADA	ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
RABELADB	ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
RABSTOP3	ELISA Stop Solution (Item I)
RABTMB3	ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
RABWASH4	20X Wash Buffer (Item B)

安全信息

象形图
警示用语：	Warning
危险声明	H290
预防措施声明	P234 - P390
危险分类	Met. Corr. 1
储存分类代码	8A - Combustible, corrosive hazardous materials
WGK	WGK 3