DNA Polymerase I fromEscherichia colilysogenic for NM 964

半岛bd体育手机客户端 说明

一般描述

DNA polymerase I (holoenzyme) has 5′→3′ and 3′→5′ exonuclease activities in addition to its synthetic activity. This bifunctional activity enables the enzyme to use nicks or gaps in double stranded DNA as starting points for DNA synthesis. The 5′→3′ exonuclease activity degrades the DNA strand complementary to the template strand beginning at the nick. DNA synthesis begins at the 3′-end of the nick and produces a new strand of DNA complementary to the template. The net result is the movement of the polymerase along the template strand (nick translation) until the DNA complementary to the template (from the site of the original nick to the 5′-end of the template strand) is replaced.

应用

Suitable for:

• Highly specific DNA probes by nick translation
• In vitro synthesis of complementary cDNA strand
• In vitro synthesis of DNA
• Produce blunt ends from 5′ and 3′ overhangs

DNA Polymerase I from Escherichia colihas been used to study the effects of the anti-tumor drug cis-diaminedichloroplatinum (II) on the enzyme activity.

组分

DNase Polymerase I is supplied in a solution of 50% glycerol containing 100 mM potassium phosphate buffer (pH 6.5), and 1 mM dithiothreitol.

单位定义

One unit converts 10 nanomoles of deoxyribonucleoside triphosphates into acid insoluble material in 30 min at 37 ℃.

基本信息

酶学委员会(EC)编号	2.7.7.7 ( BRENDA | IUBMB )
MDL编号	MFCD00130924
NACRES	NA.53

半岛bd体育手机客户端 性质

质量水平	200
等级	for molecular biology
形式	buffered aqueous glycerol solution
分子量	109 kDa
浓度	5,000-15,000 units/mL
UniProt登记号	P00582
异质活性	Endonuclease, none detected
运输	wet ice
储存温度	?20℃
Gene Information	Escherichia coliK12 ... polA(948356)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)