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Fumarase from porcine heart

品牌
Sigma-Aldrich
CAS
9032-88-6
货号
F1757
规格纯度
ammonium sulfate suspension, 300-500 units/mg protein (biuret)
价格
6367.06 *本价格含增值税费
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半岛bd体育手机客户端 名称:
9032-88-6
Fumarase from porcine heart
L -Malate hydrolyase
Fumarate hydratase
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.
Fumarase is mapped to human chromosome 1q43. Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.

应用

Fumarase from porcine heart has been used:

  • as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay
  • for immobilized enzyme based stability assay
  • in fumarate hydratase assays in human lung carcinoma cells (A549)

Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.

生化/生理作用

Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.
Fumarase is essential for regulation of cellular metabolism. Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation. Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome.

单位定义

One unit will convert 1.0 μmole of L -malate to fumarate per min at pH 7.6 at 25 ℃.

外形

Suspension in 3.2 M (NH4)2SO4, 0.05 M KH2PO4, pH 7.5, 0.014 M 2-mercaptoethanol

制备说明

Isolated by the method of Beeckmans, S., and Kanarek, L.,Eur. J. Biochem.,78, 437 (1977). Affinity purified.

基本信息

酶学委员会(EC)编号 4.2.1.2 ( BRENDA | IUBMB )
EC 号 232-887-7
MDL编号 MFCD00131111
NACRES NA.54

半岛bd体育手机客户端 性质

质量水平 200
形式 ammonium sulfate suspension
specific activity 300-500 units/mg protein (biuret)
异质活性 malic dehydrogenase ≤0.1%
储存温度 2-8℃

安全信息

象形图 GHS07
警示用语: Warning
危险声明 H317
预防措施声明 P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
危险分类 Skin Sens. 1
储存分类代码 10 - Combustible liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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