半岛bd体育手机客户端 说明
一般描述
Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.
Fumarase is mapped to human chromosome 1q43. Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.
应用
Fumarase from porcine heart has been used:
生化/生理作用
Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.
Fumarase is essential for regulation of cellular metabolism. Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation. Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome.
单位定义
One unit will convert 1.0 μmole of
外形
Suspension in 3.2 M (NH4)2SO4, 0.05 M KH2PO4, pH 7.5, 0.014 M 2-mercaptoethanol
制备说明
Isolated by the method of Beeckmans, S., and Kanarek, L.,Eur. J. Biochem.,78, 437 (1977). Affinity purified.
基本信息
酶学委员会(EC)编号 | 4.2.1.2 ( BRENDA | IUBMB ) |
EC 号 | 232-887-7 |
MDL编号 | MFCD00131111 |
NACRES | NA.54 |
半岛bd体育手机客户端 性质
质量水平 | 200 |
形式 | ammonium sulfate suspension |
specific activity | 300-500 units/mg protein (biuret) |
异质活性 | malic dehydrogenase ≤0.1% |
储存温度 | 2-8℃ |
安全信息
象形图 | |
警示用语: | Warning |
危险声明 | H317 |
预防措施声明 | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
危险分类 | Skin Sens. 1 |
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich