Monoclonal Anti-Gelsolin antibody produced in mouse

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-Gelsolin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes of an immunized mouse. Gelsolin (GSN) is encoded by the gene mapped to human chromosome 9q33.2. GSN is a calcium dependent actin binding protein. It has been shown to exist in at least two variant forms, cytoplasmic gelsolin and plasma gelsolin. Plasma gelsolin has also been called as brevin. GSN is characterized with six domains G1-G6 at N-terminal end, each domain contains Ca²⁺binding site.

特异性

The antibody is specific for an epitope located on the 47 kDa peptide derived from a chymotryptic cleavage of gelsolin. The antibody reacts with plasma and cytoplasmic gelsolin. An additional application for this antibody is immunoaffinity chromatography, for purification of gelsolin, and for detection of gelsolin-actin complexes in cell extracts.

免疫原

human plasma gelsolin.

应用

Monoclonal Anti-Gelsolin antibody produced in mouse has been used in immunoprecipitation and immunohistochemical staining.

生化/生理作用

Gelsolin (GSN) is a potent modulator of actin filament length and gelation. It binds to the plus′ ends of actin monomers and filaments, preventing monomer exchange. GSN plays a vital role in regulation of actin dynamics. Mutations in this gene cause familial amyloidosis Finnish type (FAF) characterized by cranial neuropathy and lattice corneal dystrophy. Plasma gelsolin is used as a biochemical marker for acute rheumatic carditis.

目标描述

Gelsolin is an actin-severing protein found in mammalian cells and blood plasma. Has tumor suppressor activity in several cancer cell lines and in nude mice.

其他说明

This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200750 Anti-Gelsolin antibody, Mouse Monoclonal
clone GS-2C4, purified from hybridoma cell culture

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD00164595
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	GS-2C4, monoclonal
包含	15 mM sodium azide
species reactivity	pig, rabbit, human, bovine
technique(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using heat-induced antigen retrieval sections immunohistochemistry (frozen sections): suitable indirect ELISA: suitable microarray: suitable western blot: 1:1,000 using human plasma
同位素/亚型	IgG1
UniProt登记号	P06396
运输	dry ice
储存温度	?20℃
Gene Information	human ... GSN(2934)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable