Monoclonal Anti-β-COP antibody produced in mouse

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti- β-COP (mouse IgG1 isotype) is derived from the M3A5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. COPs (coatomer proteins) have a molar mass of 110 kDa and its primary structure is homologous to the β-adaptin component of clathrin-coated vesicles.
The coatomer (approx. 550kDa) consists of proteins designated α-, β-, γ-, and δ-COP, together with substoichiometric amounts of several other proteins.

特异性

The antibody recognizes an epitope shared by β-COP (110 kDa) found in most tissue culture lines, and by a doublet of brain microtubule-associated protein (MAP2, 270-300 kDa). The antibody stains a reticular structure in the perinuclear area of non-neuronal cells (the periphery of Golgi complex and a population of coatomers scattered throughout the cytoplasm) in tissues from different species and cell processes, as well as cell bodies in chicken brain neuronal cells. It has been used for studies on the effects of various agents that influence energy status, disrupt the Golgi complex, or alter the activity of G-proteins or small GTP-binding proteins on the cellular localization of β-COP. The antibody may be used for the immunoaffinity purification of the protein.

免疫原

microtubule-associated protein from goose brain.

应用

Monoclonal Anti-β-COP antibody produced in mouse has been used:

• for the localization of β-COP using immunoprecipitation
• in immunocytochemistry
• in immunoblotting
• with other antibodies to Golgi proteins to study the role and relationships of this protein in the cell

生化/生理作用

COPs (coatomer proteins) are transiently attached to the vesicles involved in transport within the Golgi complex and possibly between the rough ER and Golgi complex.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD00164606
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	M3A5, monoclonal
包含	15 mM sodium azide
species reactivity	bovine, monkey, rabbit, goose, hamster, rat, human, canine, chicken, kangaroo rat
technique(s)	immunocytochemistry: suitable immunoprecipitation (IP): suitable indirect immunofluorescence: 1:20 using cultured Chinese hamster ovary (CHO) cells
同位素/亚型	IgG1
UniProt登记号	P53618
运输	dry ice
储存温度	?20℃
Gene Information	human ... COPB1(1315) rat ... Copb1(114023)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable