Streptavidin-Maleimide fromStreptomyces avidinii

半岛bd体育手机客户端 说明

一般描述

Streptavidin conjugated compounds may be used in avidin/strepavidin:biotin-based detection systems.
Streptavidin is a protein produced by the bacteriumStreptomyces avidinii. It has four binding sites for biotin, as does avidin. Both have been used extensively as probes in immunochemical systems, conjugated to antibodies, enzymes or fluorochromes. However, avidin is highly basic (pI ~ 10.5), compared to streptavidin (pI ~ 5-6). Avidin has a slightly higher binding capacity for biotin than streptavidin does, but it has glycosylated side chains which give higher backgrounds in blotting techniques than does streptavidin, which is not a glycoprotein. Streptavidin is more resistant than avidin to dissociation into subunits by guanidinium chloride.

应用

Maleimide Activated Streptavidin is used to prepare streptavidin-protein conjugates with proteins, peptides and other molecules that contain a free sulfhydryl (-SH) group. Activated streptavidin presents an available maleimide group that reacts with sulfhydryl-containing molecules.

外形

含柠檬酸钠的冻干粉。

制备说明

Streptavidin, fromStreptomyces avidinii, activated with maleimidocaproic acid N-hydroxysuccinimide ester.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD00274720
NACRES	NA.46

半岛bd体育手机客户端 性质

质量水平	200
标记范围	4-10 mol maleimide per mol Streptavidin
适用性	suitable for direct conjugation to compounds containing free sulfhydryl groups
储存温度	?20℃

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)