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Nitrotetrazolium blue chloride

2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride

品牌
Sigma-Aldrich
CAS
298-83-9
货号
93862
规格纯度
90.0-110.0% (calc. on dry substance, T)
价格
768.31 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
298-83-9
Nitrotetrazolium blue chloride
3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2 H-tetrazolium chloride]
2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride
p-Nitrotetrazolium blue
Nitro BT
NBT
p-Nitro-Blue tetrazolium chloride
氯化四唑氮蓝
硝基蓝四氮唑
化硝基四氮唑蓝
硝基蓝四氮唑
四唑蓝
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

生化/生理作用

Nitrotetrazolium blue chloride (NBT) is used in staining, visualizing and evaluating the damage in brain sections or heart tissues. The principle of the technique is that the redox enzymes like nicotinamide adenine dinucleotide NAD(H) and cofactors present in the cell convert colorless tetrazolium salt to blue formazan. The dead cells remain unstained. It is used in the dual-staining along with methyl green for the hyperspectral microscopic imaging analysis of colon tissues for the diagnosis of colon diseases. NBT is also used for diagnosing microbial infections and phagocytic defects. In conventional chromogenic methods, NBT/ 5-bromo-4-chloro-3-indolyl phosphate (BCIP) is used as a substrate for alkaline phosphatase enzyme reaction, in which NBT is reduced to insoluble purple diformazan.

分析说明

solubility:
250 mg in 5 mL methanol: yellow to very dark yellow and light brown-yellow to very dark brown-yellow

基本信息

线性分子式 C40H30N10O6· 2Cl
分子量 817.64
Beilstein 4115923
MDL编号 MFCD00012159
PubChem化学物质编号 329770281
NACRES NA.54

半岛bd体育手机客户端 性质

质量水平 100
测定 90.0-110.0% (calc. on dry substance, T)
杂质 ≤10% solvent content
≤10% water
λ in methanol
紫外吸收 λ: 252-262 nm Amax
储存温度 2-8℃
SMILES string [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
InChI 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
InChI key FSVCQIDHPKZJSO-UHFFFAOYSA-L

安全信息

象形图 GHS07
警示用语: Warning
危险声明 H302 - H315 - H319 - H335
预防措施声明 P261 - P264 - P270 - P301 + P312 - P302 + P352 - P305 + P351 + P338
危险分类 Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
靶器官 Respiratory system
储存分类代码 11 - Combustible Solids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

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