您好,欢迎来到半岛电竞官方网址 ! [ 登录] [ 免费注册]
半岛电竞官方网址
位置: 首页> 品牌> Sigma-Aldrich> 抗-VSV糖蛋白单克隆抗体

Monoclonal Anti-VSV Glycoprotein-Cy3TMantibody produced in mouse

抗-VSV糖蛋白单克隆抗体

品牌
Sigma-Aldrich
货号
C7706
规格纯度
clone P5D4, purified immunoglobulin, buffered aqueous solution
价格
5001.78 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Monoclonal Anti-VSV Glycoprotein-Cy3 TMantibody produced in mouse
Monoclonal Anti-VSV Glycoprotein
抗-VSV糖蛋白单克隆抗体
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-VSV Glycoprotein (mouse IgG1 isotype) is derived from the P5D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide of vesicular stomatitis virus glycoprotein (VSV-G), conjugated to keyhole limpet hemocyanin (KLH). VSV-G is a protein expressed on the surface of bullet shaped virions.

特异性

该抗体可识别包含VSV糖蛋白的五个羧基末端氨基酸的表位。在受感染的细胞中,该抗体将未成熟型VSV-G定位在粗糙内质网(RER)和高尔基复合体潴泡中,将成熟型VSV-G定位在细胞表面和出芽病毒中。该抗体不会染色缺少膜和细胞质结构域的分泌型VSV-G。该抗体可用于微注射抗体、免疫印迹、免疫沉淀、免疫细胞化学和免疫电镜法研究新合成的VSV-G在向质膜和细胞表面转移过程中细胞质结构域的作用。该抗体可用于对以VSV-G羧基末端标记的外源性导入结构进行检测、免疫沉淀和免疫细胞化学染色。该标记不会干扰所研究蛋白质的功能,可被P5D4抗体特异性识别,而不与任何内源性蛋白质发生交叉反应。

免疫原

KLH标记的含有水泡性口炎病毒糖蛋白(VSV-G)的15个羧基末端氨基酸(497-511)的合成肽。

应用

A dilution of 1:1000 of Monoclonal Anti-VSV Glycoprotein-Cy3?antibody produced in mouse was used to detect VSV glycoprotein in pig sperms by immunofluorescence.
Monoclonal Anti-VSV Glycoprotein Cy3?antibody produced in mouse has been used:

  • in studies applying
  • microinjection of antibody
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry
  • immunoelectron microscopy.

生化/生理作用

The envelope of vesicular stomatitis virus (VSV) consists of a bilayer membrane with a single type of glycoprotein, the G-protein (VSV-G) which mediates attachment to the cell surface and induces pH-dependent fusion between viral and target membranes. The carboxyl terminus of the VSV-G protein which does not have any homology with cellular proteins, has been engineered into expression vectors as a tag. Proteins expressed with this tag may thus be detected and localized using an antibody reactive specifically against this epitope with no risk of cellular background staining.
The vesicular stomatitis virus (VSV) glycoprotein facilitates the entry of HIV into the CD4 T cells through endocytotic pathway. The coupling of Cy3 to Anti-VSV Glycoprotein antibody allows for the visualization of protein by fluorescent microscopy.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

法律信息

Cy is distributed under license from Amersham Biosciences Limited.
Cy is a registered trademark of Cytiva
Cy3 is a trademark of Cytiva

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES NA.46

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
偶联物 CY3 conjugate
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 P5D4, monoclonal
形式 buffered aqueous solution
technique(s) direct immunofluorescence: 1:10,000 using COS-7 cells transfected with a VSV-G tagged vinculin construct
同位素/亚型 IgG1
运输 wet ice
储存温度 2-8℃

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK nwg
闪点(F) Not applicable
闪点(C) Not applicable
个人防护装备 Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map