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位置: 首页> 品牌> Sigma-Aldrich> Monoclonal Anti-Rabbit Immunoglobulins–Biotin antibody produced in mouse

Monoclonal Anti-Rabbit Immunoglobulins–Biotin antibody produced in mouse

品牌
Sigma-Aldrich
货号
B3275
规格纯度
clone RG-16, purified immunoglobulin, buffered aqueous solution
价格
2886.46 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
Monoclonal Anti-Rabbit Immunoglobulins–Biotin antibody produced in mouse
Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

特异性

Binds only rabbit Igs. Does not react with reduced rabbit Igs.

应用

The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps fromE. colicultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

生化/生理作用

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgA antibody is secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. IgM antibodies are present as pentamers in the serum and are produced in response to antigens.

外形

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES NA.46

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
偶联物 biotin conjugate
抗体形式 purified immunoglobulin
antibody product type secondary antibodies
克隆 RG-16, monoclonal
形式 buffered aqueous solution
species reactivity rabbit
should not react with goat, bovine, human, canine, chicken, horse, feline, sheep, rat, turkey, guinea pig, pig
human
technique(s) direct ELISA: 1:60,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,500
western blot: 1:200,000-1:400,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)
western blot: 1:200,000
同位素/亚型 IgG1
运输 dry ice
储存温度 ?20℃

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK nwg
闪点(F) Not applicable
闪点(C) Not applicable

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