您好,欢迎来到半岛电竞官方网址 ! [ 登录] [ 免费注册]
半岛电竞官方网址
位置: 首页> 品牌> Sigma-Aldrich> Monoclonal Anti-Rabbit Immunoglobulins-Alkaline Phosphatase antibody produced in mouse

Monoclonal Anti-Rabbit Immunoglobulins-Alkaline Phosphatase antibody produced in mouse

品牌
Sigma-Aldrich
货号
A2306
规格纯度
clone RG-16, purified immunoglobulin, buffered aqueous glycerol solution
价格
3234.32 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Monoclonal Anti-Rabbit Immunoglobulins-Alkaline Phosphatase antibody produced in mouse
Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-Rabbit Immunoglobulins (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulins is composed of two heavy (H) and two light (L) chains. Each containing one NH2-terminal variable region and one or more COOH-terminal constant regions. They are heterodimeric proteins.
Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

特异性

No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, sheep or turkey.
Binds only rabbit Igs. Does not react with reduced rabbit Igs.

应用

The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps fromE. colicultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.
Monoclonal Anti-Rabbit Immunoglobulins Alkaline Phosphatase antibody produced in mouse has been used in:

  • indirect enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunohistochemistry

生化/生理作用

The constant domains of immunoglobulins are involved in the complement activation.
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins.
Mouse monoclonal anti-rabbit immunoglobulins-alkaline phosphatase antibody binds to an epitope on the heavy chain of rabbit IgG, IgA, and IgM. The product stains bands at the intact whole molecule and at the heavy chains in an immunoblot of denatured, non-reduced rabbit immunoglobulins. Reduction of rabbit immunoglobulins destroys the epitope. In ELISA, the product shows no cross reaction with human serum or tissue preparations, nor with human IgA, IgG, or IgM.

外形

Solution in 0.05 M Tris buffer pH 8.0, containing 1 mM MgCl2, 0.1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
偶联物 alkaline phosphatase conjugate
抗体形式 purified immunoglobulin
antibody product type secondary antibodies
克隆 RG-16, monoclonal
形式 buffered aqueous glycerol solution
species reactivity rabbit
should not react with goat, bovine, human, canine, chicken, horse, feline, sheep, rat, turkey, guinea pig, pig
technique(s) direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 using human tonsil and Cat. No. I8635 as the primary antibody
western blot: 1:160,000-1:320,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per well)
同位素/亚型 IgG1
运输 wet ice
储存温度 2-8℃

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable
个人防护装备 Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map