半岛bd体育手机客户端 说明
一般描述
Monoclonal Anti-Plectin (mouse IgG1 isotype) is derived from the 7A8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Plectin is an abundant, high molecular weight, cytomatrix protein (300 kDa) found in a wide variety of tissue and cell types. Plectin is found in stratified and non-stratified epithelia, fibroblasts, endothelial cells and astrocytes, as well as in striated, smooth and cardiac muscle, but not in neurons.
特异性
The antibody recognizes an epitope located in the middle section of the rod domain of the plectin molecule, approximately halfway between the globular end domains. The kinase C phosphorylation site was found on the same terminal segment as this antibody epitope. The antibody specifically labels the 300 kDa band of plectin, however, lower M.W. band(s) may also be detected. The antibody stains filamentous structures on immunohistochemical staining of frozen tissue sections. The antibody inhibits the interaction of plectin with vimentin and lamin B. An additional application for this antibody is immunoelectronmicroscropy.
免疫原
plectin from rat glioma C6 cells
应用
Monoclonal Anti-Plectin antibody produced in mouse has been used in:
生化/生理作用
Plectin plays a role in the cross-linking of intermediate filaments, the interlinking of intermediate filaments with microtubules and microfilaments and the anchoring of intermediate filaments to the plasma membrane and the nuclear membrane. It is a part of the signal transduction mechanism involving kinases A and C, becausein vitroas well asin vivophosphorylation of the protein by those kinases differentially affected its binding affinities to vimentin and lamin B.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD00164718 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | ascites fluid |
| antibody product type | primary antibodies |
| 克隆 | 7A8, monoclonal |
| 分子量 | antigen 300 kDa |
| 包含 | 15 mM sodium azide |
| species reactivity | marsupial (Ptoorous tridactylisPtK2cell line), rat |
| technique(s) | dot blot: suitable immunocytochemistry: suitable immunohistochemistry (frozen sections): suitable indirect ELISA: suitable indirect immunofluorescence: 1:200 using unfixed frozen sections of rat heart microarray: suitable western blot: suitable |
| 同位素/亚型 | IgG1 |
| 运输 | dry ice |
| 储存温度 | ?20℃ |
| Gene Information | rat ... Plec1(64204) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
m.cnreagent.com
