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位置: 首页> 品牌> Sigma-Aldrich> 单克隆抗-磷酸酪氨酸

Monoclonal Anti-Phosphotyrosine-Biotin antibody produced in mouse

单克隆抗-磷酸酪氨酸

品牌
Sigma-Aldrich
货号
B1531
规格纯度
clone PT-66, purified immunoglobulin, buffered aqueous solution
价格
7114.41 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Monoclonal Anti-Phosphotyrosine-Biotin antibody produced in mouse
Phospho-Tyr
p-Tyr
Phospho-tyrosine
Monoclonal Anti-Phosphotyrosine
单克隆抗-磷酸酪氨酸
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-Phosphotyrosine (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediates numerous signalling pathways in both prokaryotic and eukaryotic cells . Cellular proteins with phosphorylated tyrosine increase many fold by the activation of tyrosine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain serine/threonine/tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. Monoclonal anti-phosphotyrosine?biotin antibody can be used in dot blot (diluted 1:32,000). Mouse anti-phosphotyrosine?biotin antibody reacts specifically with phosphorylated tyrosine both as the free amino acid or when conjugated to BSA or KLH. This product does not react with non-phosphorylated tyrosine or other phosphorylated amino acids, including serine and threonine, or with phosphorylated molecules like AMP or ATP.
经 ELISA 和竞争性 ELISA 确定,该抗体与磷酸化的酪氨酸特异性反应,该磷酸化的酪氨酸既可以是游离氨基酸形式,也可与 BSA 或 KLH 等载体结合的形式。与非磷酸化的酪氨酸、磷酸苏氨酸、磷酸丝氨酸、AMP 或 ATP 没有观察到交叉反应。

免疫原

与 BSA 结合的磷酸酪氨酸

应用

Monoclonal Anti-Phosphotyrosine?Biotin antibody produced in mouse has been used in western blot analysis to detect tyrosine phosphorylated proteins. It has also been used in BIAcore analysis. This conjugate maybe used as an analytical tool by enabling the identification and quantification of tyrosine phosphorylated proteins.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES NA.44

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
偶联物 biotin conjugate
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 PT-66, monoclonal
形式 buffered aqueous solution
technique(s) direct ELISA: 1:50,000
dot blot: 1:32,000
同位素/亚型 IgG1
运输 dry ice
储存温度 ?20℃

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK nwg
闪点(F) Not applicable
闪点(C) Not applicable

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