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Monoclonal Anti-β1and β2-Adaptins antibody produced in mouse

Monoclonal Anti-β1and β2-Adaptins antibody produced in mouse

品牌
Sigma-Aldrich
货号
A4450
规格纯度
clone 100/1, ascites fluid
价格
4731.57 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Monoclonal Anti-β 1and β 2-Adaptins antibody produced in mouse
Anti-AP-1 and AP-2
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti- β1 and β2-Adaptins (AP-1 & AP-2)(mouse IgG1 isotype) is derived from the 100/1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Adaptins AP-1 and AP-2 are mapped to human chromosome 22q12.2 and 17q12, respectively. AP-1 adaptor consists of four subunits. It includes two large adaptins (one each of γ/α/δ/ε and β1?4 , one medium adaptin (μ1?4), and one small adaptin (?1?4. Similarly, the AP-2 is made up of two 100-110 kDa polypeptides, termed α and β2 (formerly β) subunits, F2 , and s2 subunit. α and β2 subunits (α and β 2).

免疫原

AP-2 adaptor protein from bovine brain.

应用

Monoclonal Anti-β1 and β2-Adaptins antibody produced in mouse has been used in immunoblotting and immunohistology.
Mouse monoclonal anti-β1 and β2-adaptins can be used for western blot (1:200) assays using rat brain extract. The antibody is also suitable for use in indirect immunofluorescence and microarray analyses. Furthermore, monoclonal anti-β1/β2-adaptins have been used for double staining methods to detect clathrin-coated regions of the plasma membrane in rat brain cells.
Immunofluorescence of MDCK cells fixed in methanol was performed using anti-AP1 at a dilution of 1:250 and also western blot at a dilution of 1:1000.

生化/生理作用

Clathrin-coated vesicles facilitate the selective internalization of membrane receptors for lysosomal enzymes from the trans-Golgi network to a pre-lysosomal compartment. Besides clathrin, coated vesicle populations contain the adaptor complexes AP-1 and AP-2, also known as HA-I (HA1) adaptor and HA-II (HA2) adaptor or Assemble Protein 1 (AP1) and Assemble Protein 2 (AP2), respectively. These adaptor proteins mediate the interaction between clathrin and membrane components.
β1and β2 adaptins are highly homologous which suggests that the β -type adaptor chains may mediate the interaction of their adaptors with the highly conserved clathrin molecule. Monoclonal antibodies reacting specifically against adaptor proteins are useful tools for studies on the intracellular distribution and structural relationship of adaptor complexes.

外形

The antibody is provided as ascites fluid with 0.1% sodium azide as a preservative.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号 MFCD00212883
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
偶联物 unconjugated
抗体形式 ascites fluid
antibody product type primary antibodies
克隆 100/1, monoclonal
包含 15 mM sodium azide
species reactivity bovine, rat, human
technique(s) indirect immunofluorescence: suitable
microarray: suitable
western blot: 1:200 using rat brain extract
同位素/亚型 IgG1
UniProt登记号 P63010
Q10567
运输 dry ice
储存温度 ?20℃
Gene Information human ... AP1B1(162), AP2B1(163)
rat ... Ap1b1(29663), Ap2b1(140670)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

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