半岛bd体育手机客户端 说明

一般描述

该抗体可识别在所有肌动蛋白亚型中均保守的C末端表位。它能够特异性标记各种组织和物种中的肌动蛋白。 被抗体识别的表位能够耐受甲醛固定和石蜡包埋。锌-福尔马林、B5、乙醇、methacarn、Brunnel's或Bouin's溶液也可用作固定液。
Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Actin has six isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. There are three α-actins (α-skeletal, α-cardiac and α-smooth muscle), one β-actin (β-nonmuscle) and two γ-actins (γ-smooth muscle and γ-nonmuscle).

免疫原

与多抗原肽(MAP)骨架连接的合成肌动蛋白C末端肽

应用

Monoclonal Anti-Actin-Cy3 antibody has been used in immunofluorescent staining.
肌动蛋白单克隆抗体被用于Cos-7细胞裂解物的蛋白质免疫印迹分析，以确保等量蛋白质上样。
已使用肌动蛋白单克隆抗体作为一抗，对MDCK细胞裂解物进行蛋白质免疫印迹分析。

生化/生理作用

Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

其他说明

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

法律信息

Distributed under license from Amersham Biosciences Limited.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	CY3 conjugate
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	AC-40, monoclonal
形式	buffered aqueous solution
分子量	antigen 42 kDa
species reactivity	human, carp,Xenopus, canine, sheep, pig, mouse, rabbit, rat, hamster, chicken, snail, bovine, viper, guinea pig, goat
technique(s)	direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts
同位素/亚型	IgG2a
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable