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位置: 首页> 品牌> Sigma-Aldrich> 抗-山羊/绵羊 IgG-碱性磷酸酶单克隆抗体 小鼠抗

Anti-Goat/Sheep IgG-Alkaline Phosphatase antibody, Mouse monoclonal

单克隆抗 山羊/绵羊 IgG

品牌
Sigma-Aldrich
货号
A8062
规格纯度
clone GT-34, purified from hybridoma cell culture
价格
3099.26 *本价格含增值税费
促销
服务
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数量
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半岛bd体育手机客户端 名称:
Anti-Goat/Sheep IgG-Alkaline Phosphatase antibody, Mouse monoclonal
Monoclonal Anti-Goat/Sheep IgG
Monoclonal Anti-Goat/Sheep IgG–Alkaline Phosphatase antibody produced in mouse
抗-山羊/绵羊 IgG-碱性磷酸酶单克隆抗体 小鼠抗
单克隆抗 山羊/绵羊 IgG
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

IgGs are glycoprotein antibodies that modulate several immune responses. Primary goat against target proteins are often used in various research applications. Thus, secondary anti-goat IgGs can be useful tools for the analysis of target proteins. However, secondary antibodies may lack species specificity as they may bind to non-related Igs in the test preparation. This may lead to increased levels of background staining. Hence, use of monoclonals can effectively reduce background staining and increase the specificity of immunoassays.
Monoclonal Anti-Goat/Sheep IgG-Alkaline Phosphatase recognizes goat and sheep IgGs. No cross-reactivity is observed with human IgGs or IgGs derived from the following species: guinea pig, rat, horse, dog, chicken, pig, mouse, rabbit, and cat.
抗体与位于山羊 IgG1 和 IgG2 重链上的表位反应。

免疫原

Purified goat IgG

应用

The level of IL-5 in HPAEC conditioned medium was determined by ELISA using alkaline phosphatase-conjugated monoclonal mouse anti-goat/sheep IgG (GT-34) as the secondary antibody.
Mouse monoclonal anti-goat/sheep IgG-alkaline phosphatase antibody has been used as a secondary antibody for ELISA assays at 1:4,000 and 1:15,210 dilutions. The product can also be used for IHC (1:40) and western blot applications (1:150,000).

外形

Solution in 0.05 M Tris buffer pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

生物来源 mouse
偶联物 alkaline phosphatase conjugate
抗体形式 purified from hybridoma cell culture
antibody product type secondary antibodies
克隆 GT-34, monoclonal
形式 buffered aqueous glycerol solution
species reactivity bovine, sheep, goat
should not react with rat, chicken, mouse, horse, feline, pig, canine, guinea pig, rabbit, human
technique(s) direct ELISA: 1:50,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40
western blot (chemiluminescent): 1:150,000 using total cell extract of chicken fibroblasts (10-20 μg per well)
同位素/亚型 IgG1
运输 wet ice
储存温度 2-8℃

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable
个人防护装备 Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

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