半岛bd体育手机客户端 说明
一般描述
IgGs are glycoprotein antibodies that modulate several immune responses. Primary goat against target proteins are often used in various research applications. Thus, secondary anti-goat IgGs can be useful tools for the analysis of target proteins. However, secondary antibodies may lack species specificity as they may bind to non-related Igs in the test preparation. This may lead to increased levels of background staining. Hence, use of monoclonals can effectively reduce background staining and increase the specificity of immunoassays.
Monoclonal Anti-Goat/Sheep IgG-Alkaline Phosphatase recognizes goat and sheep IgGs. No cross-reactivity is observed with human IgGs or IgGs derived from the following species: guinea pig, rat, horse, dog, chicken, pig, mouse, rabbit, and cat.
抗体与位于山羊 IgG1 和 IgG2 重链上的表位反应。
免疫原
Purified goat IgG
应用
The level of IL-5 in HPAEC conditioned medium was determined by ELISA using alkaline phosphatase-conjugated monoclonal mouse anti-goat/sheep IgG (GT-34) as the secondary antibody.
Mouse monoclonal anti-goat/sheep IgG-alkaline phosphatase antibody has been used as a secondary antibody for ELISA assays at 1:4,000 and 1:15,210 dilutions. The product can also be used for IHC (1:40) and western blot applications (1:150,000).
外形
Solution in 0.05 M Tris buffer pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
半岛bd体育手机客户端 性质
生物来源 | mouse |
偶联物 | alkaline phosphatase conjugate |
抗体形式 | purified from hybridoma cell culture |
antibody product type | secondary antibodies |
克隆 | GT-34, monoclonal |
形式 | buffered aqueous glycerol solution |
species reactivity | bovine, sheep, goat |
should not react with | rat, chicken, mouse, horse, feline, pig, canine, guinea pig, rabbit, human |
technique(s) | direct ELISA: 1:50,000 immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 western blot (chemiluminescent): 1:150,000 using total cell extract of chicken fibroblasts (10-20 μg per well) |
同位素/亚型 | IgG1 |
运输 | wet ice |
储存温度 | 2-8℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich