Phosphomannose Isomerase fromEscherichia coli

半岛bd体育手机客户端 说明

应用

PMI is used to study cell wall synthesis and energy production. PMI has been used to study how EDTA and metal ions, such as Zn++, Co++, Fe++, Mn++ and Cu++., can affect recovery and thermal stability. It may be used to study PMI′s effect on various alginate biosynthetic enzymes such as phosphomannomutase (PMM), GDP-mannose pyrophosphorylase (GMP), and GDP-mannose dehydrogenase (GMD).

生化/生理作用

Phosphomannose Isomerase (PMI) catalyses the interconversion of mannose 6-phosphate (Man-6-P) and fructose 6-phosphate (Fru-6-P), which provides a link between glucose metabolism and mannosylation.

包装

Bottomless glass bottle. Contents are inside inserted fused cone.

单位定义

One unit will convert 1.0 μmole of D -mannose 6-phosphate to D -fructose 6-phosphate per min at pH 7.6 at 25 ℃, using a coupled enzyme system with phosphoglucose isomerase and glucose-6-phosphate dehydrogenase.

外形

Supplied as a suspension in 3.2 M ammonium sulfate

基本信息

酶学委员会(EC)编号	5.3.1.8 ( BRENDA | IUBMB )
MDL编号	MFCD00131899
NACRES	NA.54

半岛bd体育手机客户端 性质

质量水平	200
重组	expressed inE. coli
形式	ammonium sulfate suspension
specific activity	≥50 units/mg protein
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)