半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-Vimentin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Vimentin is expressed in breast myoepithelial cells, osteocytes, Langerhans cells of the skin, Schwann cells and astrocytes.
Vimentin is one of the five major groups of intermediate filaments with a molecular mass of 57 kDa. Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological and biochemical criteria. IFs differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments and are components of most eukaryotic cells.

特异性

在免疫组织化学染色中，抗体将波形蛋白定位于成纤维细胞、内皮细胞、淋巴组织和黑色素细胞中。它也在肉瘤、淋巴瘤和黑色素瘤中染色波形蛋白。

免疫原

猪眼晶状体波形蛋白。

应用

Monoclonal Anti-Vimentin-Cy3 antibody produced in mouse has been used in:

• immunofluorescence and confocal microscopy
• cytoskeleton staining
• double labeling experiments
• immunohistochemical
• immunocytochemical localozation

生化/生理作用

Cy3 Monoclonal Anti-Vimentin reacts with normal and pathological tissue of mesenchymally-derived cells, lens tissue and various cultured cells. It localizes vimentin in fibroblasts, lipocytes, endothelial cells, some lymphoid cells, melanocytes, macrophages, and chondrocytes. The antibody stains tumors derived from these cells, including sarcomas, most lymphomas, melanomas and their metastatic lesions. Co-expression of vimentin with keratin was described in most carcinomas of certain sites (e.g., kidney and thyroid) and certain carcinomas of other sites (e.g., breast).
Vimentin was found to be important for stabilizing the architecture of the cytoplasm. It was found, that activated macrophages secrete vimentin into the extracellular spacein vitro.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

储存及稳定性

Store at 2-8 ℃. Protect from prolonged exposure to light. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

法律信息

Cy is distributed under license from Amersham Biosciences Limited.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	CY3 conjugate
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	V9, monoclonal
形式	buffered aqueous solution
分子量	antigen ~58 kDa
species reactivity	chicken, rat, bovine, feline, hamster, gerbil, horse, canine, rabbit, monkey, pig, human
technique(s)	direct immunofluorescence: 1:200 using cultured cells immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using human tonsil immunohistochemistry (frozen sections): suitable using human tonsil
同位素/亚型	IgG1
UniProt登记号	P08670
运输	wet ice
储存温度	2-8℃
Gene Information	human ... VIM(7431) rat ... Vim(81818)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)