半岛bd体育手机客户端 说明
一般描述
Photoreceptors are responsible for the initial step in visual processing, converting the signal of photon absorption into synaptic transmission. The visual pigment in vertebrate rods responsible for the absorption of light quanta is rhodopsin (also called opsin). Rhodopsin comprises >95% of the rod outer segments (ROS) intrinsic membrane protein and is a glycoprotein possessing two asparagine-linked oligosaccharide groups at amino acid residues 2 and 15 of the N-terminus. Light is absorbed by rhodopsin and the subsequent conformational change leads to the activation of a cyclic GMP phosphodiesterase through a specific G-protein intermediate, transducin.
Mouse monoclonal clone RET-P1 anti-Opsin antibody recognizes an epitope located in amino acid residues 4-10 at the N-terminus of the rhodopsin molecule. In immunoblotting of rat retina, the antibody labels a closely spaced doublet of 39 kDa and less intense bands of 78 and 115 kDa, representing rhodopsin monomer and aggregates. Higher M.W. bands are stained in pituitary (251 and 288 kDa) and in hypothalamus (269 and 331 kDa) in addition to several lower M.W. bands. The product specifically labels the cell bodies, outer and inner segments (rods but not cones) of rat photoreceptor surface. It reacts with bovine, duck, amphibian, goldfish, rabbit, rat, turtle, tiger salamander, mouse, dove, quail, and human rhodopsin/opsin.
Opsin belongs to rhodopsin receptor. The gene is located on human chromosome 3q22.1. Opsin gene is mainly expressed in rod cells.
免疫原
rat retinal membranes.
应用
Mouse monoclonal clone RET-P1 anti-Opsin antibody is used to tag opsin/rhodopsin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting, immunocytochemistry of cultured cells, immunohistochemistry (paraformaldehyde/glutaraldehyde-fixed, paraformaldehyde perfusion-fixed, frozen sections), immunoelectron microscopy, ELISA, competitive ELISA, and solid phase RIA. It is used as a probe to determine the presence and roles of opsin/rhodopsin in studies of rhodopsin location, functional properties and molecular mechanisms governing rod photoreceptor differentiation.
Monoclonal Anti-Opsin antibody produced in mouse has been used in immunofluorescence and histology.
生化/生理作用
Mutations in rhodopsin gene are linked to retinitis pigmentosa (RP) and congenital night blindness.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
MDL编号 | MFCD01865244 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 200 |
生物来源 | mouse |
偶联物 | unconjugated |
抗体形式 | ascites fluid |
antibody product type | primary antibodies |
克隆 | RET-P1, monoclonal |
分子量 | antigen 39 kDa by immunoblotting (IB of rat retina produces closely spaced doublet) |
包含 | 15 mM sodium azide |
species reactivity | bovine, quail, dove, rat, tiger salamander, amphibian, rabbit, human, goldfish, turtle, mouse, duck |
technique(s) | electron microscopy: suitable immunocytochemistry: suitable using cultured cells immunohistochemistry (frozen sections): 1:10,000 using frozen sections of rat eye indirect ELISA: suitable radioimmunoassay: suitable western blot: suitable |
同位素/亚型 | IgG1 |
UniProt登记号 | P08100 |
运输 | dry ice |
储存温度 | ?20℃ |
Gene Information | human ... RHO(6010) mouse ... Rho(212541) rat ... Rho(24717) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |