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一般描述

与人IgG结合；不与其他人Igs结合。该抗体对人IgG重链上的决定簇具有特异性。在3％ PEG 6000存在时，该抗体可在液相中形成抗原-抗体复合物。
Monoclonal Anti-Human IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulins have two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. About 70 percent of the total immunoglobulin consists of IgG.

应用

利用碱性磷酸酶偶联的小鼠抗-人IgG单克隆抗体通过Elisa对健康个体血清中针对各种金黄色葡萄球菌抗原的抗体进行检测。将抗体在PBSt中按1:30000稀释，并在37度下与样品孵育2小时。Reactin是使用对硝基苯基磷酸酯底物(Sigma)进行开发的。
Monoclonal Anti-Human IgG (γ-chain specific) antibody produced in mouse has been used in enzyme linked immunosorbent assay (ELISA).

生化/生理作用

Immunoglobulin G (IgG) mainly helps in immune defense. IgG participates in hypersensitivity type II and type III.

免责声明

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