半岛bd体育手机客户端 说明

一般描述

该抗体可使用竞争性ELISA识别游离的生物素和生物胞素。通过使用间接ELISA或免疫组织化学染色，该抗体还可识别与各种免疫球蛋白耦合的生物素。特异性是通过使用生物素化山羊抗体与涂覆在多孔板上的人和兔抗原而评估的。
Monoclonal Anti-Biotin (mouse IgG1 isotype) is derived from the BN-34 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Biotin is a naturally occurring vitamin and co-enzyme required by cells in living organisms or in culture.

免疫原

生物素-KLH偶联物

应用

Monoclonal Anti-Biotin?Cy3^?antibody produced in mouse has been used in:

• fluorescent microscopy
• RNA fluorescencein situhybridization (FISH){36
• immunostaining

该抗体可被用于扩增技术、ELISA、免疫印迹、 原位核酸杂交、流式细胞术、图像分析或共聚焦显微镜。
在一些应用中，生物素化探针与亲合素的定位会产生高背景水平。抗生物素试剂可代替亲和素以降低非特异性结合。

生化/生理作用

Cy dyes are pH insensitive fluorophores that are used to label, DNA, RNA and proteins in a variety of biological assays.
Monoclonal Anti-Biotin- Cy3 can be used in many other applications where biotin can be introduced as target label. It can be used in detection of DNA and mRNA by sensitive non-isotopicin situhybridization. It may also be used for detection of microinjected biotin-haptenized cytoskeletal proteins allowing examination of the pattern of incorporation and turnover of cytoskeletal proteins in living cells.

外形

Solution in phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

法律信息

Cy is distributed under license from Amersham Biosciences Limited.
Cy3 is a trademark of Cytiva

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.46

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	CY3 conjugate
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	BN-34, monoclonal
形式	buffered aqueous solution
technique(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 indirect immunofluorescence: 1:200
同位素/亚型	IgG1
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)