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位置: 首页> 品牌> Sigma-Aldrich> 单克隆抗肌动蛋白抗体

Monoclonal Anti-Actin–FITC antibody produced in mouse

单克隆抗肌动蛋白抗体

品牌
Sigma-Aldrich
货号
F3046
规格纯度
clone AC-40, purified immunoglobulin, buffered aqueous solution
价格
4731.57 *本价格含增值税费
促销
服务
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  • 增值税票
数量
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半岛bd体育手机客户端 名称:
Monoclonal Anti-Actin–FITC antibody produced in mouse
Monoclonal Anti-Actin
单克隆抗肌动蛋白抗体
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

该抗体可识别在所有肌动蛋白亚型中均保守的C末端表位。它能够特异性标记各种组织和物种中的肌动蛋白。 被抗体识别的表位能够耐受甲醛固定和石蜡包埋。锌-福尔马林、B5、乙醇、methacarn、Brunnel's或Bouin's溶液也可用作固定液。
Monoclonal Anti-Actin (mouse IgG2a isotype) is derived from the AC-40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. It has one N-terminal region which appears to be a major antigenic region and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.

免疫原

与多抗原肽(MAP)骨架连接的合成肌动蛋白C末端肽

应用

肌动蛋白单克隆抗体被用于Cos-7细胞裂解物的蛋白质免疫印迹分析,以确保等量蛋白质上样。
已使用肌动蛋白单克隆抗体作为一抗,对MDCK细胞裂解物进行蛋白质免疫印迹分析。
Actin–FITC antibody is suitable for direct immunofluorescence at a dilution of 1:75, using cultured human or chicken fibroblasts.

生化/生理作用

Actin, a conserved eukaryotic protein, is the major cytoskeletal component which actively participates in cell motility. It is associated with myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. In mammals and birds, actin is expressed as six different isoforms. It has one N-terminal region which appears to be a major antigenic region, and may be involved in the interaction of actin with other proteins such as myosin. Actin isoforms show 50-60% homology in their 18 N-terminal residues.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

其他说明

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
偶联物 FITC conjugate
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 AC-40, monoclonal
形式 buffered aqueous solution
分子量 antigen 42 kDa
species reactivity human, carp,Xenopus, canine, sheep, pig, mouse, rabbit, rat, hamster, chicken, snail, bovine, viper, guinea pig, goat
technique(s) direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts
同位素/亚型 IgG2a
运输 dry ice
储存温度 ?20℃

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK nwg
闪点(F) Not applicable
闪点(C) Not applicable

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