Duolink^?flowPLA Compensation Beads-Green

半岛bd体育手机客户端 说明

应用

Duolink^?proximity ligation assay(PLA^?) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

It is recommended that Duolink^?compensation beads should be used when performing a multiplexed experiment (DUO96001) to save on time and reagent cost, rather than using precious sample. Please note that these Green beads are specific to the Green fluor utilized in the Duolink^?Multicolor FlowPLA kit, not the Green fluorophore in the stand-alone Duolink^?microscopy and flow kits. This product can be used to determine flow cytometry instrumentation collection parameters, inform experimental design, and as a control for compensation data for multiplexed FlowPLA experiments.

Visit our Duolink^?PLA Resource Center for information on how to run a Duolink^?experiment, applications, troubleshooting, and more.

Duolink^?Multicolor flow PLA Reagent Pack Kits used with Duolink^?PLA Multicolor Probemaker Kits will enable Multiplexing of up to 4 protein events with sensitive detection of proteins, protein-protein interactions, and protein modifications within cell populations by flow cytometry. To perform a Duolink^?flowPLA experiment, you will need fixed and permeabilized suspended cells, and at least two primary antibodies that specifically recognize your proteins of interest. Analysis is carried out using standard flow cytometry assay equipment. User must provide a fixed cell suspension and primary antibodies. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^?in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). Flow validated antibodies are recommended.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink^?projects

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特点和优势

• Save time and reagent cost by utilizing beads for compensation rather than precious sample and multiplex reagents
• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

组分

This product is comprised of the following:
6.0 - 8.0μm polystyrene beads suitable for use in flow cytometry assays. The bead slurry should have a concentration of 1.5-3.0 million beads/mL. Green beads can be mixed with the negative control compensation (DUO84010), or collected alone.

法律信息

Duolink is a registered trademark of sigma-aldrich Co. LLC
PLA is a registered trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

质量水平	200
半岛bd体育手机客户端 线	Duolink?
technique(s)	flow cytometry: suitable immunocytochemistry: suitable immunofluorescence: suitable multiplexing: suitable proximity ligation assay: suitable
荧光	λex498 nm; λem523 nm (excitation laser line 488 nm)
适用性	suitable for fluorescence
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable