半岛bd体育手机客户端 说明
一般描述
Synaptotagmin (Syt, p65) is an abundant synaptic vesicle (SV) membrane protein. It is characterized by a short intravesicular N-terminal domain, a single transmembrane region and two copies of highly conserved internal repeats, known as the C2A and C2B domains, which are homologous to the C2 regulatory region of protein kinase C (PKC) in the cytoplasmic domain. At least eight different isoforms of synaptotagmin (SytI-VIII) are expressed in the brain, four of which (Syt IV, V, VII and VIII) are also expressed in non-neuronal tissues.
特异性
The sequence is highly conserved among species (SytI) and is not found in other known synaptotagmin isoforms (SytII-VIII).
免疫原
synthetic peptide corresponding to N-terminus of synaptotagmin I (SytI) of rat origin (amino acids 1-16 with C-terminally added lysine), conjugated to KLH.
应用
Anti-Synaptotagmin antibody produced in rabbit has been used:
生化/生理作用
Synaptotagmin binds Ca2+phospholipids with high affinity and has a central role in Ca2+regulated neurotransmitter release. Synaptotagmin functions as a Ca2+sensor and is required for efficient exocytosis, particularly in the vesicle docking and/or fusion step with the plasma membrane. Ca2+influx triggers synaptotagmin to interact with either syntaxin or SNAP-25 and the cytoplasmic domain of neurexin leading to fusion and exocytosis. Mutations or deletion of synaptotagmin result in severely impaired Ca2+triggered neurotransmitter release. Synapses of SytI knockout mice lack the fast-component of Ca2+dependent neurotransmitter release, but exhibit no changes in the slow, Ca2+independent component of synaptic vesicle exocytosis.
外形
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
MDL编号 | MFCD01092496 |
NACRES | NA.46 |
半岛bd体育手机客户端 性质
生物来源 | rabbit |
质量水平 | 200 |
偶联物 | unconjugated |
抗体形式 | IgG fraction of antiserum |
antibody product type | primary antibodies |
克隆 | polyclonal |
形式 | buffered aqueous solution |
分子量 | antigen 65 kDa |
species reactivity | rat |
technique(s) | microarray: suitable western blot: 1:5,000 using extract of rat brain membrane fraction |
UniProt登记号 | P21707 |
运输 | dry ice |
储存温度 | ?20℃ |
Gene Information | human ... SYT1(6857) mouse ... Syt1(20979) rat ... Syt1(25716) |
Sigma-Aldrich