Anti-PAK3 antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

p21-activated kinases are a family of highly conserved serine/threonine protein kinases that are activated by small GTPases of Cdc42 and Rac families. There are 2 groups of PAKs in mammals, group A and B. The group A members are three isoforms, PAK1, PAK2, and PAK3 (also known as α-PAK, γ-PAK, and β-PAK, respectively). PAKs are actively involved in regulation of MAPK pathways by phosphorylation of Raf-1 and MEK-1. They also regulate cytoskeletal reorganization by interaction with GTPases. Autophosphorylation of threonine 423 (threonine 402 for PAK2 and threonine 421 for PAK3), catalyzed by Cdc42, is required for activation of PAK.
Anti-PAK3 specifically reacts with mouse and rat PAK3 (65 kDa). It reacts weakly with human PAK3.

免疫原

synthetic peptide corresponding to amino acids 1-13 of the N-terminal of mouse p21 activated kinase 3 (PAK3).

应用

Anti-PAK3 may be used at a concentration of 4 μg to immunoprecipitate PAK3 from 0.5-1 mg mouse NIH3T3 total cell lysate. It may be used at a concentration of 1-2 μg/mL for detection in total mouse NIH3T3 lysate by immunoblotting.

外形

Solution in 0.07 M Tris-glycine, pH 7.4, containing 30% glycerol and 0.035% sodium azide.

基本信息

MDL编号	MFCD01633961
NACRES	NA.44

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	200
偶联物	unconjugated
抗体形式	IgG fraction of antiserum
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous glycerol solution
分子量	antigen 65 kDa
species reactivity	mouse, rat
technique(s)	immunoprecipitation (IP): 4 μg using 0.5 mg of mouse brain cytosol western blot (chemiluminescent): 2 μg/mL using mouse 3T3 fibroblasts extract or mouse brain extract
UniProt登记号	Q62829
运输	wet ice
储存温度	?20℃
Gene Information	mouse ... Pak3(18481) rat ... Pak3(29433)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter