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位置: 首页> 品牌> Sigma-Aldrich> Anti-MSK-1 antibody produced in rabbit

Anti-MSK-1 antibody produced in rabbit

品牌
Sigma-Aldrich
货号
M5437
规格纯度
IgG fraction of antiserum, buffered aqueous solution
价格
4692 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Anti-MSK-1 antibody produced in rabbit
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

The mitogen- and stress-activated kinases, MSK-1 and MSK-2 are activated by ERK/MAPK and p38 MAPK in response to growth and cell stress stimuli. MSK-1 and MSK-2 have 75% homology and interact with ERK1/2 and p38 through MAPK-binding domains near the C-terminus. There is a predominant expression of MSK proteins in heart, brain, skeletal muscles, lung, liver, kidney, pancreas and placenta. Highly conserved phosphorylation sites of MSK-1, Ser260 and Thr581, are followed by proline residues indicating that MSK-1 is activated by more than one MAPK. Endogenous MSK-1 is activated in cells by growth factors, phorbol ester stimulation, exposure to UV irradiation, and oxidative or chemical stress and is localized to nucleus of unstimulated or stimulated cells. MSK-1 is directly involved in the regulation of gene expression since it actively phosphorylates, stabilizes and activates many transcription factors. The major transcription factors that are regulated by MSK-1 include CREB, NF-κB and STAT3. MSKs also evidently phosphorylate Histone H3 at Ser10 and Ser28 and HMG at Ser6. Thus a primary role of MSK proteins is also promotion of gene relaxation and activation by regulating the binding of transcription repressors. Anti-MSK-1 specifically recognizes rat MSK-1 (90 kDa).

免疫原

synthetic peptide corresponding to the C-terminus of human MSK1 (amino acids 783-800). This sequence is not found in the human and mouse MSK2 isoforms.

应用

Anti-MSK-1 may be used at a minimum working dilution of 1:2000 (immunoblotting in whole cell extracts) or 1:1000 (immunocytochemistry) in fibroblast Rat1 cell line. The antobody has also been used for detection by immunoblotting in mouse fibroblast 10T1/2 cells.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号 MFCD02686432
NACRES NA.44

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 200
偶联物 unconjugated
抗体形式 IgG fraction of antiserum
antibody product type primary antibodies
克隆 polyclonal
形式 buffered aqueous solution
分子量 antigen 90 kDa
species reactivity rat
technique(s) immunocytochemistry: 1:1,000 using methanol/acetone-fixed rat fibroblast Rat-1 cell line
microarray: suitable
western blot: 1:2,000 using using whole cell lysate of rat fibroblast Rat-1 cell line
运输 dry ice
储存温度 ?20℃
Gene Information human ... RPS6KA5(9252)
rat ... Rps6ka5(445349)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

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