半岛bd体育手机客户端 说明
一般描述
Monoclonal anti-β-1-Chicken Integrin (mouse IgG1 isotype) is derived from the W1B10 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified chicken integrin from fibroblasts of 11 days old chicken embryos. Chicken integrin is a heterodimer consisting of the common β-1 subunit and two α subunits (α-5 or α-3). The β-1 subunit of chicken integrin is a 803-amino acid transmembrane glycoprotein consisting of a large extracellular domain and a short cytoplasmic domain.
特异性
The antibody recognizes an epitope on the extracellular side of the avian integrin complex, and inhibits adhesion of embryo and tendon fibroblasts as well as skeletal myoblasts to both laminin and fibronectin. Under reducing and denaturing conditions using extracts of chicken embryo muscle cells, the antibody precipitates the β1class integrin heterodimer as a broad band of ~140 kDa. In the absence of reducing agents, the integrin β1subunit appears as bands at 100 kDa (immature form), 100 kDa (mature form), and 140 kDa (noncovalently associated α-subunits). The antibody may also be used for bioactivity studies in adhesion assays.
免疫原
chicken integrin.1
应用
Anti-Integrin β1 antibody, Mouse monoclonal has been used in western blot, fluorescent activated cell sorter (FACS) analysis.
生化/生理作用
Integrin binding to extracellular matrix (ECM) ligands regulates cell adhesion, shape, motility, intracellular signalling and gene expression. The monoclonal anti-b-1 chicken integrin antibody (clone W1B10) has been used to inhibit chicken muscle cell adhesion in cell cultures.
外形
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin with 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD02178888 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | W1B10, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen 100-140 kDa |
| 包含 | 15 mM sodium azide |
| species reactivity | chicken |
| technique(s) | immunoprecipitation (IP): suitable indirect immunofluorescence: suitable western blot: 1:200 using denatured, non-reduced extract of cultured fibroblasts from chicken embryo lower leg |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P07228 |
| 运输 | dry ice |
| 储存温度 | ?20℃ |
| Gene Information | chicken ... ITGB1(374058) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
