TargeTron^TMVector pJIR750ai

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一般描述

TargeTron^?vector pJIR750ai is a 10,262 bp expression vector intended for use with the TargeTron Gene Knockout System, Catalog Number TA0100. This circularized vector can be used for targeted gene knockouts in gram-positive bacteria such asClostridium perfringenstype A.¹Expression of the group II intron RNA is under the control of the β-2 toxin gene promoter,cpb2.¹As supplied, the pJIR750ai vector is re-targeted to the α-toxin (plc) gene. α-Toxin, a phospholipase C, is an extracellular toxin produced by allC. perfringensisolates.¹For validation in a specificC. perfringensstrain, this vector can be used directly to knockoutplcwithout any further modifications using the enclosed protocol. In order to re-target this vector to knockout other genes, theplcspecific IBS-EBS fragment (350 bp) between theHindIII andBsrG I sites can be cut out and replaced with another gene specific fragment. For re-targeting protocols, refer to the TargeTron Gene Knockout System User Guide, Catalog Number TA0100, at sigma-aldrich.com.

法律信息

TargeTron is a trademark of InGex, LLC

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安全信息