半岛bd体育手机客户端 说明
一般描述
Monoclonal Anti-VEGF Receptor-1 (Flt1 Receptor) (mouse IgG1 isotype) is derived from the FLT-19 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant extracellular domain of VEGF Receptor-1 (Flt1 Receptor) of human origin. Vascular endothelial growth factor (VEGF), also called vasculotropin (VAS)1 and vascular permeability factor (VPF) 2 is a member of a family of endothelial cell mitogens and angiogenic factors. VEGF is a homodimeric heparin-binding glycoprotein. VEGF binds to two structurally similar receptor tyrosine kinases; Flt15 (fms-like tyrosine kinase 1, also termed VEGF Receptor-1, VEGF-R1) and KDR6 (kinase-insert domain containing receptor, also termed VEGF-R2). Flt1 is predominately expressed in human placenta and human vascular endothelial cells, while KDR is more widely expressed in all vessel-derived endothelial cells but low in human and fetal bovine placenta.
Mouse monoclonal clone FLT-19 anti-Vascular Endothelial Growth Factor Receptor-1 antibody recognizes the extracellular domain of human VEGF Receptor-1 molecule (Flt1 Receptor). The antibody does not recognize VEGF Receptor-2 (KDR), VEGF receptor-3 (sFlt4,) and PDGF-Rβ.
免疫原
recombinant human extracellular domain of VEGFR-1.
应用
Mouse monoclonal clone FLT-19 anti-Vascular Endothelial Growth Factor Receptor-1 antibody may be used for the localization of VEGF-R1 using various immunochemical assays such as ELISA, immunoblotting, immunocytochemistry, immunohistochemistry, and immunoprecipitation. Antibodies that react specifically with VEGF receptors are useful for the study of the specific differential tissue expression and intracellular localization of the receptor in normal and neoplastic tissue.
Monoclonal Anti-Vascular Endothelial Growth Factor Receptor-1 antibody produced in mouse has been used in:
生化/生理作用
Vascular endothelial growth factor (VEGF) stimulates the proliferation of endothelial cells isolated from both small and large vessels.
The mitogenic activity of VEGF appears to be stimulated by specific VEGF receptors (160-200 kDa) which can be found on the surface of various endothelial cells. VEGF binds to two structurally similar receptor tyrosine kinases; Flt1 (fms-like tyrosine kinase 1, also known as VEGF Receptor-1 (VEGF-R1), and KDR (kinase insert domain containing receptor, also known as VEGF-R2). Studies using KDR and Flt1 stably transfected endothelial cell lines have shown that these two receptors exhibit different affinities to VEGF and mediate different responses. KDR/Flk1 does not respond to placental growth factor (PlGF), a VEGF related growth factor, while Flt1 binds PlGF specifically. Flt1 is predominately expressed in human placenta and human vascular endothelial cells. Both VEGF receptors (KDR and Flt1) are upregulated in human fetal and adult kidney.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD01634354 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | tissue culture supernatant |
| antibody product type | primary antibodies |
| 克隆 | FLT-19, monoclonal |
| 包含 | 15 mM sodium azide |
| species reactivity | human |
| technique(s) | immunohistochemistry (frozen sections): 1:100 using human placenta immunoprecipitation (IP): suitable indirect ELISA: suitable microarray: suitable |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | P17948 |
| 运输 | dry ice |
| 储存温度 | ?20℃ |
| Gene Information | human ... FLT1(2321) |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H373 |
| 预防措施声明 | P260 - P314 - P501 |
| 危险分类 | STOT RE 2 |
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
Sigma-Aldrich
m.cnreagent.com
