Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse

半岛bd体育手机客户端 说明

一般描述

Activated/ Monophosphorylated (Phosphothreonine ERK-1&2) (mouse IgG1 isotype) is derived from the ERK-PT115 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Mitogen-activated protein kinase 1 (MAPK1) is mapped to human chromosome 22q11.22 and MAPK3 is present on human chromosome 16p11.2.

特异性

The antibody reacts specifically with the monophosphorylated threonine and the active diphosphorylated forms of MAP kinase (ERK-1 and ERK-2). It does not recognize the non-phosphorylated form of the MAP kinase molecule, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated threonine residue within the regulatory site of active MAP kinase (e.g.,Thr¹⁸³in ERK-2).

免疫原

synthetic peptide HTGFLpTEYVAT, corresponding to the phosphorylated form of ERK-activation loop.

应用

Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse has been used in:

• immunoblotting
• western blotting
• immunofluorescence
• enzyme linked immunosorbent assay (ELISA)
• immunochemistry

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD01633612
NACRES	NA.44

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	ERK-PT115, monoclonal
形式	buffered aqueous solution
分子量	antigen, ERK-1 44 kDa antigen, ERK-2 42 kDa
species reactivity	human, rat
浓度	~2 mg/mL
technique(s)	capture ELISA: suitable immunocytochemistry: suitable microarray: suitable western blot: 0.5 μg/mL using extract of a rat fibroblast cell line, Rat1, activated with sorbitol
同位素/亚型	IgG1
UniProt登记号	P28482
运输	dry ice
储存温度	?20℃
Gene Information	human ... MAPK1(5594), MAPK3(5595) rat ... Mapk1(116590), Mapk3(50689)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable