Anti-MAP Kinase, Monophosphorylated Tyrosine antibody ,Mouse monoclonal

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-MAP Kinase, Mono-phosphorylated Tyrosine (pY-ERK) (mouse IgG1 isotype) is derived from the ERK-PY193 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide sequence corresponding to the phosphorylated form of ERK-activation loop, conjugated to KLH. The mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in many signaling pathways. This family includes the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK (c-Jun N-terminal protein kinase, also termed stress-activated protein kinase, SAPK1), and p38 MAPK (also termed SAPK2) subfamilies.

特异性

The antibody reacts with the monophosphorylated tyrosine form of MAP kinases (ERK1 and ERK2). It does not recognize the non-phosphorylated, diphosphorylated, and the monophosphorylated threonine forms of ERK/MAP kinases, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated tyrosine residue within the regulatory site of MAP kinase (e.g.,Tyr¹⁸⁵in ERK-2). Cross-reactivity has been observed with the monophosphorylated tyrosine peptide of JNK.

免疫原

synthetic peptide HTGFLTEpYVAT, corresponding to the phosphorylated form of ERK-activation loop.

应用

Monoclonal Anti-MAP Kinase, Monophosphorylated Tyrosine antibody has been used in:

• immunoblotting
• enzyme linked immunosorbent assay (ELISA)
• dot-blot
• immunocytochemistry
• western blotting
• immunofluorescence

生化/生理作用

MAPK kinase (MAPKK) is the immediate upstream activator of the MAPK, MAPKK kinase (MAP3K), and MAP3K kinase (MAP4K) for the enzymes further upstream, respectively. The kinases in the MAPK level are activated by phosphorylation of both tyrosine (Y) and threonine (T) residues organized in a TXY motif. The residue in between the two phosphorylated residues determines the specificity of activation of the MAPKs. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs.

外形

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

制备说明

Prepared from a culture supernatant of bioreactor grown hybridoma.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号

MFCD02096848

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	purified from hybridoma cell culture
antibody product type	primary antibodies
克隆	ERK-PY193, monoclonal
形式	buffered aqueous solution
分子量	antigen, ERK-1 44 kDa antigen, ERK-2 42 kDa
species reactivity	rat, human
浓度	~2 mg/mL
technique(s)	capture ELISA: suitable immunocytochemistry: suitable microarray: suitable western blot: 1-5 μg/mL using cell extract of rat fibroblasts cell line, Rat1, activated with sorbitol
同位素/亚型	IgG1
UniProt登记号	P28482
运输	dry ice
储存温度	?20℃
Gene Information	human ... MAPK1(5594), MAPK3(5595) rat ... Mapk1(116590), Mapk3(50689)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable