Anti-Epidermal Growth Factor antibody, Mouse monoclonal antibody produced in mouse

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-Epidermal Growth Factor (EGF) (mouse IgG1 isotype) is derived from the EGF-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Epidermal growth factor (EGF) is a single chain polypeptide hormone (6 kDa). EGF protein exists largely in a β-sheet conformation, with a C-terminal hairpin that may be involved in receptor binding. Human EGF is found in body fluids such as urine, milk, saliva, sweat, and seminal fluid and has its highest concentration in α-granules of blood platelets.

特异性

Specifically reacts with both native and recombinant human EGF. The antibody does not cross-react with mouse EGF.

免疫原

recombinant human EGF

应用

Monoclonal Anti-Epidermal Growth Factor antibody produced in mouse was used to study rat immune cells by flow cytometry to detect the presence or absence of hormone and hormone-like molecules in response to EGF treatment.
Monoclonal Anti-Epidermal Growth Factor antibody has been used in dot-blot, radioimmunoassay, enzyme linked immunosorbent assay (ELISA), immunohistology, immunoblotting, immunostaining.

生化/生理作用

EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.
Epidermal growth factor (EGF) is a potent growth-promoting factor for a variety of tissue cellsin vivoandin vitro. EGF binding induces phosphatidylinositide hydrolysis and generation of diacylglycerol (DAG). Cellular metabolic effects of EGF include stimulation of ion fluxes, glucose transport, glycolysis and synthesis of DNA, RNA and proteins. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells. The biological role of EGF includes the inhibition of gastric acid secretion, support of growth and differentiation during fetal development, neuromodulation in the central nervous system and stimulation of epidermal growth and keratinization.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD00239669
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	purified from hybridoma cell culture
antibody product type	primary antibodies
克隆	EGF-10, monoclonal
形式	buffered aqueous solution
分子量	antigen 6 kDa
species reactivity	human
technique(s)	dot blot: 0.1 μg/mL using recombinant human EGF immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using human tissue indirect ELISA: suitable microarray: suitable neutralization: suitable radioimmunoassay: suitable western blot: suitable
同位素/亚型	IgG1
UniProt登记号	P01133
application(s)	research pathology
运输	dry ice
储存温度	?20℃
Gene Information	human ... EGF(1950)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves