半岛bd体育手机客户端 说明
一般描述
Monoclonal Anti-Cdc27 (mouse IgG2b isotype) is derived from the AF3.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to C-terminal of human Cdc27, conjugated to KLH.
The gene CDC27 (Cell division cycle protein 27 homolog) is mapped to human chromosome 17q12–23.2. The protein localizes in the nucleus.
免疫原
synthetic peptide corresponding to the C-terminus 10 residues of human Cdc27.
应用
Monoclonal Anti-Cdc27 antibody produced in mouse is suitable for the following applications:
生化/生理作用
The Cdc16/27/34 complex or the Anaphase-promoting complex/cyclosome (APC/C) complex functions as an ubiquitin conjugating enzyme, ubiquitinating cyclin B, and resulting in cyclin B/Cdk complex degradation.
CDC27 (Cell division cycle protein 27 homolog) is a component of anaphase-promoting complex/cyclosome (APC/C), an E3 ligase involved in cell cycle control. Phosphorylation of CDC27 is crucial for tumor growth factorβ (TGFβ)-induced activation of APC. It interacts with Microcephalin (MCPH1), causative gene for primary recessive autosomal microcephaly. DNA-damage response mediator MDC1 interacts with APC/C through CDC27. Down-regulation of CDC27 in irradiated cervical cancer cell line results in greater survival fraction. Polymorphism in CDC27 are linked with breast cancer.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD01633250 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | AF3.1, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen 97 kDa |
| species reactivity | rat, bovine, human, canine,Xenopus, mouse |
| 浓度 | ~2 mg/mL |
| technique(s) | immunocytochemistry: suitable using 4% paraformaldehyde fixation, Triton X-100 permeabilization immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable immunoprecipitation (IP): suitable indirect ELISA: suitable microarray: suitable western blot: 1-2 μg/mL using a HeLa Cell nuclear extract |
| 同位素/亚型 | IgG2b |
| UniProt登记号 | P30260 |
| 运输 | dry ice |
| 储存温度 | ?20℃ |
| Gene Information | human ... CDC27(996) mouse ... Cdc27(217232) rat ... Cdc27(360643) |
Sigma-Aldrich
m.cnreagent.com
