Anti-Amyloid Precursor Protein, N-Terminal antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

Amyloid precursor proteins (APPs) are transmembrane glycoproteins that are found in a wide range of tissues. APPs have 3 main isoforms, namely, APP695, APP751 and APP770 that are derived from alternative splicing events in cells. It is expressed at high levels in the brain. APP gene is mapped to human chromosome 21q11.2-q21. It is a 695 amino acid protein which possesses a large ectodomain and comparatively short intracellular region.
The immunogen sequence is identical to the APP isoforms APP₇₅₁and APP₇₇₀and is highly conserved (single amino acid substitution) in rat and mouse APP₆₉₅. The antibody recognizes APP₆₉₅, APP₇₅₁and APP₇₇₀.

免疫原

synthetic peptide corresponding to the N-terminal of human APP₆₉₅(amino acids 46-60) conjugated to KLH.

应用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Anti-Amyloid Precursor Protein, N-Terminal antibody produced in rabbit has been used in:

• western blotting
• immunostaining
• immunofluorescence

SH-SY5Y cell lysates were analyzed by western blot using rabbit anti-Amyloid Precursor Protein, C-Terminal as the primary antibody at a 1:500 dilution.

生化/生理作用

Amyloid precursor proteins (APPs) regulates cell growth, motility, neurite outgrowth and cell survival. The intracellular C-terminus of APP serves as a transcriptional regulator and as a receptor for kinesin-1-mediated axonal transport. Alzheimer′s disease is characterized by deposition of amyloid in the central nervous system, in neurite plaques and on cerebral vasculature. Mutations in the APP gene are linked with rare forms of autosomal dominant familial Alzheimer′s Disease (FAD). APPs undergo post-translational processing includingN- andO-glycosylation, phosphorylation and sulfation.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD02095830
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	200
偶联物	unconjugated
抗体形式	IgG fraction of antiserum
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous solution
分子量	antigen 95-100 kDa
species reactivity	human, mouse, rat
technique(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formic acid-treated sections of human Alzheimer′s disease (AD) brain microarray: suitable western blot: 1:1,000 using rat brain extract or supernatant of 293T cells secreting APP.
UniProt登记号	P05067
运输	dry ice
储存温度	?20℃
Gene Information	human ... APP(351) mouse ... App(11820) rat ... App(54226)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable