KAPA2G Fast PCR Kit

半岛bd体育手机客户端 说明

一般描述

KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets.
KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.

应用

KAPA2G Fast PCR Kit has been used in:

• PCR with confronting 2 -pair primers assay (PCR-CTPP)
• Routine PCR
• Fast PCR
• Genotyping

生化/生理作用

KAPA2G Fast DNA Polymerase generated DNA fragments is useful in sequencing restriction enzyme digestion and cloning experiments. KAPA2G Fast has 5′→3′ polymerase and 5′→3′ exonuclease activities like wild-typeTaq, but no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is similar to that of wild-typeTaq; it has an error rate of approximately 1 error per 1.7 x 10⁵nucleotides incorporated.

特点和优势

Reduce cycling times up to 75% :

• Extension times as low as 1 sec per kilobase
• Broad coverage of both AT- and GC-rich targets

High speed, higher performance :

• Single copy sensitivity from complex targets
• Increased yields

Quick Notes :

• KAPA2G Fast PCR Kits with dNTPs contain the engineered KAPA2G Fast DNA Polymerase, developed for fast PCR.
• Use 1 sec extension time for amplicons <1 kb and 15 sec/kb for longer amplicons, and save 20–70% of total reaction time.
• No need for specialized instrumentation or PCR consumables.
• Optimized buffer system offers improved yields, specificity and sensitivity, facilitating efficient primer annealing across a wide range of primer lengths, GC contents, and melting temperatures.
• Use 0.5 U KAPA2G Fast DNA Polymerase per 25 μL reaction, or less for smaller volumes.
• For high reaction efficiency, do not exceed 25 μL reaction volumes.
• KAPA2G Buffer A contains 1.5 mM MgCl₂at 1X.
• Reaction products are 3′-dA-tailed and may be cloned into TA cloning vectors.

质量

Each batch of KAPA2G Fast DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA2G Fast Ready Mix is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.

制备说明

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4℃ for short-term use (up to 1 month). Return to -20℃ for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4℃ is not recommended. Please note that reagents stored at temperatures above -20℃ are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.

其他说明

For Research Use Only. Not for use in diagnostic procedures.

基本信息

NACRES

NA.54

半岛bd体育手机客户端 性质

质量水平	100
用途	sufficient for 500 reactions
保质期	≤18 mo.
特点	Fast PCR Multiplex PCR dNTPs included hotstart: no
包装	pkg of 100 U (KK5008) pkg of 250 U (KK5009)
manufacturer/tradename	Roche
technique(s)	PCR: suitable
input	purified DNA
运输	dry ice
储存温度	?20℃

组份列表

组份不可单独销售

货号	组份
	KAPA2G Fast Standard or HotStart?DNA Polymerase (5 U/μL)
	5X KAPA2G Buffer A with MgCl2
	MgCl225 mM

安全信息

象形图
警示用语：	Warning
危险声明	H302 - H371 - H412
预防措施声明	P260 - P264 - P270 - P273 - P308 + P311 - P501
危险分类	Acute Tox. 4 Oral - Aquatic Chronic 3 - STOT SE 2
储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	does not flash
闪点(C)	does not flash