Viral RNA Extraction Buffer

半岛bd体育手机客户端 说明

一般描述

The Viral RNA Extraction Buffer has been optimized to provide rapid, room-temperature lysis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral particles in saliva. The buffer comes as a 4x working solution that can be mixed with virus-containing samples in a qRT-PCR plate. Upon 5 minute incubation at room temperature, viral particles are lysed and RNA is stabilized such that samples are ready for direct analysis via RT-qPCR with no additional cleanup step. Removal of precipitates in saliva via centrifugation prior to testing is recommended for optimal sensitivity.

应用

The Viral RNA Extraction Buffer is broadly compatible with most nucleic acid detection methods and does not reduce signal when used at working concentration in qRT-PCR. qRT-PCR compatibility has been demonstrated in a 4-fluor multiplex setting consisting of Cy5, TxRed, FAM, and HEX.
RT-qPCR compatibility has been verified with several one-step RT-qPCR kits:

• Quantitative RT-PCR ReadyMix^?(sigma-aldrich, catalog QR0200)
• KAPA PROBE FAST One-Step Universal (sigma-aldrich, catalog KK4752)
• KiCqStart^?One-Step Probe RT-qPCR ReadyMix^?(sigma-aldrich, catalog KCQS07)
• TaqPathTM 1-Step Multiplex Master Mix (Thermo Fisher, catalog A28525)

The Viral RNA Extraction Buffer is also compatible with colorimetric LAMP assays.

特点和优势

• Extracted viral RNA can be used for detection without cleanup
• Directly compatible with quantitative reverse transcription polymerase chain reaction (qRT-PCR) and loop mediated isothermal amplification(LAMP) assays
• Ideal for enveloped RNA viruses in saliva, saline, Amies medium, and viral transport medium
• Highly sensitive and faster than other leading methods, lysis by Proteinase K and heating

法律信息

KiCqStart is a registered trademark of Qiagen Beverly Inc.
ReadyMix is a trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

质量水平	200
等级	Molecular Biology
形式	liquid
浓度	4X
technique(s)	LAMP: suitable PCR: suitable RT-PCR: suitable qPCR: suitable
运输	wet ice
储存温度	?20℃

安全信息

象形图
警示用语：	Danger
危险声明	H318
预防措施声明	P280 - P305 + P351 + P338
危险分类	Eye Dam. 1
储存分类代码	10 - Combustible liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable