Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) Agarose Conjugate

半岛bd体育手机客户端 说明

一般描述

Recognizes the ~21 kDa ras protein in SW480 and Y1 cells and normal skin tissue. For neutralization studies, Cat. No. OP01L is recommended. For immunoprecipitation, Cat. No. OP01A is recommended.
This Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) Agarose Conjugate is validated for use in Immunoprecipitation, Other Applications for the detection of v-H-Ras (Ab-1).
Rat monoclonal antibody generated by immunizing Sprague Dawlay rats with the specified immunogen and fusing splenocytes with Y3Ag1.2.3 rat myeloma cells. Conjugated to agarose. Recognizes the ~21 kDa ras protein (see comments).

免疫原

recombinant p21 Ras

包装

.5 ml in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

Immunoprecipitation (15 ?l beads or 1 ?g antibody per sample, see comments)

Other Applications (see comments)

警告

Toxicity: Standard Handling (A)

外形

In PBS.

分析说明

Positive Control
SW 480 or Y1 cells or normal skin tissue

其他说明

Agarose conjugated recommended for immunoprecipitation. For frozen sections, paraffin sections, immunoblotting, neutralization, and immunofluoresence use Cat. No. OP01 or OP01L. Broadly reactive by immunoprecipitation with both phosphorylated and non-phosphorylated forms of v-H-ras and v-K-ras(see application references) and reacts with the p21 translational products of the H-, K-, and N-rashuman genes (see application references). This antibody does not cross-react with rap. Neutralizes the biological and biochemical activities of H-, K-, and N-ras p21 of vertebrates by binding to residues Glu-63, Ser-65, Ala-66, Met-67, Gln-70, and Arg-73 (see application references). 15 ?l agarose conjugate is recommended to be used as an immunoconcentrator prior to western blot analysis of p21^raswith another antibody. To immunoprecipitate with the unconjugated antibody, use Protein A with a rabbit anti-rat IgG or Protein G PLUS with a goat anti-rat IgG. Protein A alone will not immunoprecipitate rat IgG. A doublet may be detected corresponding to ras protein with and without farnesyl group. Antibody should be titrated for optimal results in individual systems.
Shimizu, K., et al. 1983.Proc. Natl. Acad. Sci. USA80, 2112.
Taparowsky, E., et al. 1983.Cell34, 581.
Ellis, R.W., et al. 1981.Nature292, 506.
Shih, T.Y., et al. 1980.Nature287, 686.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	rat
antibody product type	primary antibodies
克隆	Y13-259, monoclonal
形式	suspension (Liquid)
包含	≤0.1% sodium azide as preservative
species reactivity	rat, mouse, human
manufacturer/tradename	Calbiochem?
储存条件	do not freeze
同位素/亚型	IgG1
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable