Monoclonal Anti-β-Catenin antibody produced in mouse

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti- β -Catenin (mouse IgG1 isotype) is derived from the 6F9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. CTNNB1 (catenin β 1) gene is mapped to human chromosome 3p21. The protein encoded is also called as cadherin-associated protein.The encoded protein β-catenin is localized to the nucleus. Monoclonal antibody reacting specifically with β -catenin is an essential tool in defining the interactions and distributions of β -catenin and its relationships with other catenins and cadherins in various cells and tissues.

特异性

The antibody does not cross-react with plakoglobin (γ-catenin).

免疫原

recombinant chicken β-catenin.

应用

Monoclonal Anti-β-Catenin antibody produced in mouse has been used in:

• immunohistochemistry
• immunofluorescence
• immunoblotting

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

生化/生理作用

In Wnt/β-catenin signal pathway, β-Catenin participates as a signal transduction molecule. The pathway is associated with development, tissue homeostasis and oncogenic transcription. Upregulation of the gene is associated with glioma cell proliferation. The encoded protein has dual function that regulates the coordination of cell-cell adhesion by linking cytoskeleton and cellular junctions and gene transcription. Wnt/β-catenin signal pathway is known to induce progression of colon cancer progression. It is useful for differential diagnosis of Osteoblastoma (OB) and osteosarcoma (OS). β-catenin signaling pathway may contribute to cholangiocarcinoma (CCA) cell proliferation. Monoclonal antibody reacting specifically with β -catenin is an essential tool in defining the interactions and distributions of β -catenin and its relationships with other catenins and cadherins in various cells and tissues.

外形

Supplied as ascites fluid with 15 mM sodium azide as a preservative.

储存及稳定性

For extended storage freeze in working aliquots. For continuous use, store at 2-8 ℃. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD00282178
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	6F9, monoclonal
分子量	antigen 94 kDa
包含	15 mM sodium azide
species reactivity	human, canine, chicken, bovine
technique(s)	indirect immunofluorescence: 1:1,000 using cultured MDBK cells microarray: suitable western blot: 1:1,000 using cultured MDBK cells extract
同位素/亚型	IgG1
UniProt登记号	P35222
运输	dry ice
储存温度	?20℃
Gene Information	human ... CTNNB1(1499)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)