Anti-Collagen Type III antibody, Mouse monoclonal

半岛bd体育手机客户端 说明

一般描述

Anti-collagen type III antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the FH-7A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c immunized mouse.
Monoclonal anti-collagen type III specifically recognizes native and denatured collagen type III from human 1 and rat 2 origin. It does not recognize collagen types I, II, IV, V, VI and X.
Collagen type III, also known as collagen α-1(III) (COL3A1), is encoded by the gene mapped to human chromosome 2q24.3-q31. It is the highly expressed collagen in blood vessels and hollow organs.

免疫原

Human Collagen Type III

生化/生理作用

Collagen a-1(III) (COL3A1) serves as a potent adjunct marker for both differentiating fibroadenoma (FA) from phyllodes tumor (PT) and measuring malignant potential in PTs. Overexpression of the gene in glomerular leads to the development of collagen type III glomerulopathy. Mutations in the gene is associated with the development of familial aortic aneurysms and Ehlers-Danlos syndrome.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

储存及稳定性

Store at -20℃. For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

其他说明

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

基本信息

NACRES

NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
抗体形式	purified from hybridoma cell culture
antibody product type	primary antibodies
克隆	FH-7A, monoclonal
形式	buffered aqueous solution
分子量	~70 kDa
species reactivity	human, rat
浓度	~1.0 mg/mL
technique(s)	ELISA: suitable immunoblotting: suitable immunofluorescence: suitable immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded rat skin sections.
同位素/亚型	IgG1
UniProt登记号	P02461
运输	dry ice
储存温度	?20℃
Gene Information	human ... COL3A1(1281)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable