Anti-FOXC1 Antibody

半岛bd体育手机客户端 说明

一般描述

Forkhead box protein C1 (FOXC1) is a member of the family of forkhead transcription factors. These proteins are characterized by the forkhead domain--a 110 amino acid domain that binds to DNA molecules. FOXC1 has been implicated in the development of the eye; disruption of the FOXC1 gene has been linked to a number of glaucoma-related diseases such as Axenfeld-Rieger syndrome.

特异性

Other homologies: Rat (71% sequence homology).

免疫原

KLH-conjugated linear peptide corresponding to human FOXC1.

应用

Research Sub Category
Cell Cycle, DNA Replication & Repair
Anti-FOXC1 Antibody is a Rabbit Polyclonal Antibody for detection of FOXC1 also known as Forkhead box protein C1, Forkhead-related protein FKHL7, FREAC-3 & has been validated in WB, ICC.
Research Category
Epigenetics & Nuclear Function
Western Blot Analysis: A representative lot of this antibody detected FOXC1 in HeLa, HEK293, THP1, and MDA-MB-468 cell lysates, and in human bone marrow tissue lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected FOXC1 in HeLa cells. This antibody positively stains the nucleus, with some cytoplasm staining. This staining pattern has also been observed by an independent laboratory (Berry, F. B., et al. (2002). J Biol Chem. 277(12):10292-10297.).

质量

Evaluated by Western Blot in THP1 cell lysate.
Western Blot Analysis: 0.025 ?g/mL of this antibody detected FOXC1 in 10 ?g of THP1 cell lysate. No Tween^?reagent was used in the wash buffers and in the primary and secondary antibody dilution buffers.

目标描述

~75 kDa observed. Uniprot describes a molecular weight at ~57 kDa This protein may be observed at ~65 kDa in some cell lysates. (Tamimi, Y., et al. (2006). Hum Mol Genet. 15(21): 3229-3240.).

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
THP cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

TWEEN is a registered trademark of Croda International PLC

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human
species reactivity (predicted by homology)	mouse (based on 100% sequence homology)
technique(s)	immunocytochemistry: suitable western blot: suitable
NCBI登记号	NP_001444
UniProt登记号	Q12948
运输	wet ice
Gene Information	human ... FOXC1(2296)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable