Anti-Endostatin Antibody

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一般描述

Endostatin is a proteolytic cleavage product from the C-terminus of Collagen XVIII, which is itself a proteoglycan/collagen found in blood vessel walls and in basement membranes. Endostatin is a compact globular fold-containing protein with a Zn2+ binding site near its amino terminus. Originally identified in conditioned media of murine hemangioendothelioma (EOMA) cells, endostatin is generated from the parent collagen XVIII molecule involving intermediate glycosylated forms and the proteolytic activity of proteases such as cathepsin L, metalloproteases, and elastase. Endostatin has a wide-spread tissue expression and has been shown to co-localize with fibulins, nidogen-2 in basement membranes, and elastic fibers of veins. Endostatin has also been shown to cause a G1 arrest in endothelial cells and to induce apoptosis.

特异性

This antibody recognizes Endostatin at the nonhelical region 11 domain.

免疫原

Epitope: Nonhelical Region 11 Domain
Linear peptide corresponding to the nonhelical region 11 of mouse Endostatin.

应用

Research Category
Cell Structure
Anti-Endostatin Antibody detects level of Endostatin & has been published & validated for use in WB, IH(P).
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Endostatin in mouse lung tissue.
Research Sub Category
Adhesion (CAMs)

质量

Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Endostatin in 10 µg of A431 cell lysate.

目标描述

~28 kDa observed.
A ~28 kDa endostatin fragment has been observed in western blot as a result of MMP-7 cleavage of collagen XVIII (Chang, J., et al. (2003). Invest Ophthalmol Vis Sci. 44:832).

联系

Replaces: AB1880

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
A431 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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