半岛bd体育手机客户端 说明
一般描述
其抗体(也称为抗α-Sm-1)对α-平滑肌肌动蛋白的单一异构体具有特异性。它在免疫印迹试验中可与α-平滑肌肌动蛋白发生特异性反应,并可在冷冻或福尔马林固定的、或石蜡包埋的组织切片中对平滑肌细胞进行标记。
Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle).
免疫原
N-末端合成的α-平滑肌肌动蛋白十肽。
应用
Immunocytochemistry was performed on kertocytes fixed in 1% PFA and incubated with mouse monoclonal anti-smooth muscle actin (14A) at a 1:100 dilution.
Mouse monoclonal anti-actin, α?smooth muscle-alkaline phosphatase antibody has been used for immunohistochemical application in mouse and human tissues.
使用单克隆抗ACTA2抗体对来自牛主动脉的平滑肌细胞进行免疫细胞化学分析。首先将细胞在玻璃盖玻片上生长,并在4%的50%丙酮/乙醇中固定10分钟。
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
外形
Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide as a preservative.
其他说明
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 200 |
生物来源 | mouse |
偶联物 | alkaline phosphatase conjugate |
抗体形式 | purified from hybridoma cell culture |
antibody product type | primary antibodies |
克隆 | 1A4, monoclonal |
形式 | buffered aqueous glycerol solution |
分子量 | antigen ~42 kDa |
species reactivity | human, frog, sheep, chicken, goat, bovine, rat, guinea pig, mouse, canine, rabbit, snake |
technique(s) | ELISA: suitable immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil or appendix sections western blot: 1:100 using chicken gizzard extract/ Mouse heart extract |
同位素/亚型 | IgG2a |
UniProt登记号 | P62736 |
运输 | wet ice |
储存温度 | 2-8℃ |
Gene Information | mouse ... Acta2(11475) rat ... Acta2(81633) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich