ChemiScreen V1A Vasopressin Membrane Preparation

半岛bd体育手机客户端 说明

一般描述

Human AVPR1 cDNA encoding V_1A
Arginine vasopressin (AVP) is a 9 amino acid peptide that functions as an antidiuretic, vasoconstrictor and neurotransmitter. The three vasopressin receptors, V_1A, V_1Band V₂, are GPCRs; V_1Aand V_1Bcouple to G_qand calcium release, whereas V₂couples to G_s. V₁receptors regulate fluid homeostasis by stimulating contraction of glomerular mesangial cells and renal vascular smooth muscle cells (Birnbaumer, 2000). Chemicon′s V_1Amembrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of V_1Ainteractions with its ligands. The membrane preparations exhibit a Kd of 55 pM for [¹²⁵I]-vasopressin antagonist ([¹²⁵I]-phenylacetyl-D-Tyr(Me)-Phe-Gln-Asn-Arg -Pro-Arg-Tyr-NH2). With 10 μg/well V_1AMembrane Prep and 0.1 nM [¹²⁵]-vasopressin V_1Aantagonist, greater than 12-fold signal-to-background ratio were obtained.

应用

Radioligand binding assay, and GTPγS binding.
Human V1A GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

生化/生理作用

• GPCR Class: A
• Protein Target: V1A
• Target Sub-Family: Vasopressin

包装

1 mL, 2 mg/mL

外形

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives. Packaging method: Membranes protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80℃.

储存及稳定性

Maintain frozen at -70℃ for up to 2 years. Do not freeze and thaw.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

基本信息

eCl@ss

32161000

半岛bd体育手机客户端 性质

质量水平	100
生物来源	human
重组	expressed in Chem-1 cells
manufacturer/tradename	ChemiScreen Chemicon?
technique(s)	ligand binding assay: suitable (GTPγS) radioligand binding assay (RLBA): suitable
NCBI登记号	NM_000706.3
UniProt登记号	P37288

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable