Anti-SMN antibody, Mouse monoclonal

半岛bd体育手机客户端 说明

一般描述

Mouse monoclonal clone 2B1 anti-SMN antibody recognizes human, mouse, and Xenopus survival of motor neurons proteins.

特异性

The antibody recognizes human, mouse, and Xenopus SMN.

免疫原

recombinant human SMN

应用

Mouse monoclonal clone 2B1 anti-SMN antibody is an important tool for studying the role of the survival of motor neurons protein in nuclear processes and spinal muscular atrophy (SMA). It may be used in immunoblotting (~35 kDa), immunoprecipitation, and immunocytochemistry.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)

生化/生理作用

Survival of Motor Neurons (SMN) complex is important in various biological processes, such as assembly and restructuring of spliceosomal small nuclear ribonucleoproteins (snRNPs), pre mRNA splicing and transcription. Spinal muscular atrophy (SMA) is caused by reduced expression or mutations in the survival of motor neurons (SMN) protein. Deletion or mutation in the telomeric copy (SMN1) causes the SMA phenotype. The severity of SMA is in direct correlation with the expression level of the SMN protein, either from the SMN1 gene or a different spliced form of SMN from the SMN2 gene. The SMN complex interacts with various protein substrates such as Sm and Lsm proteins of the spliceosomal snRNPs, fibrillarin, GAR1, RNA helicase A, the human hnRNP proteins (hnRNPQ, U and R), coilin and p53.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15mM sodium azide.

储存及稳定性

For continuous use, store at 2-8 ℃ for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD01634191
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	purified from hybridoma cell culture
antibody product type	primary antibodies
克隆	2B1, monoclonal
形式	buffered aqueous solution
species reactivity	mouse,Xenopus, human
浓度	~2 mg/mL
technique(s)	microarray: suitable western blot: 2-4 μg/mL using A431 cell extract
同位素/亚型	IgG1
运输	dry ice
储存温度	?20℃
Gene Information	human ... SMN1(6606), SMN2(6607) mouse ... Smn1(20595)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable