半岛bd体育手机客户端 说明

一般描述

Calbindin- D -28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin), is a highly conserved 28 kDa calcium binding protein, with broad tissue distribution. Itbelongs , to a family of low molecular weight calcium-binding proteins (CaBPs). Calbindin- D -28K is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial cells.

免疫原

synthetic peptide corresponding to the C-terminal region of rat calbindin-D-28K (amino acids 185-199). This sequence is identical in the corresponding human, mouse and bovine calbindin-D-28K sequences and is highly conserved (single amino acid substitution) in chicken and frog calbindin-D-28K.

应用

Anti-Calbindin- D -28K (KD-15) antibody produced in rabbit has been used in:

• immunocytochemistry
• western blotting
• microarray
• immunohistochemistry

生化/生理作用

Calbindin- D -28K is an important neuroanatomical marker. Despite its broad tissue distribution, it exhibits a cell-type-specific expression pattern. It has been immunocytochemically localized in selected cells in hippocampus, where it is thought to buffer intracellular calcium, or transport intramembranous calcium.
Calbindin 1 (CALB) is a calcium-binding protein. The protein may play a role in development of Purkinje cells found in heterotopias and cerebellar dysgenesias. It also acts as a very important component of intracellular homeostasis in cerebellar neurons. The progressive decrease of calbindin content in the Purkinje cells undergoes degeneration and death during paraneoplastic changes in the cerebellum. Calbindin effects on Parkinson′s disease (PD) risk displays population specificity.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

分析说明

Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min. at RT or 37 ℃) improves immunohistochemical staining.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD00164553
NACRES	NA.44

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	200
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous solution
分子量	antigen 28 kDa
species reactivity	chicken, human, rat
technique(s)	immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using sections of rat, human or chicken cerebellum sections microarray: suitable western blot: 1:1,000 using recombinant rat calbindin-D-28K
UniProt登记号	P05937
运输	dry ice
储存温度	?20℃
Gene Information	human ... CALB1(793) mouse ... Calb1(12307) rat ... Calb1(83839)

安全信息

危险声明	H412
预防措施声明	P273
危险分类	Aquatic Chronic 3
储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable